Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing.
Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing.
复制标题
DOI：
10.4155/bio.14.200
复制
发表时间：
2014-11
期刊：
影响因子：
1.8
通讯作者：
J. Boccard;F. Badoud;Nicolas Jan;R. Nicoli;C. Schweizer;F. Pralong;J. Veuthey;N. Baume;S. Rudaz;M. Saugy
中科院分区：
文献类型：
--
作者：
J. Boccard;F. Badoud;Nicolas Jan;R. Nicoli;C. Schweizer;F. Pralong;J. Veuthey;N. Baume;S. Rudaz;M. Saugy
目的通过发现新的违禁药物滥用生物标志物，非靶向代谢组学方法有望大大有利于反兴奋剂程序。结果从十一酸睾酮摄入后的控制消除研究中监测到尿液样本中的内源性类固醇代谢产物。使用了耦合超高压LC与高分辨率四极杆TOF MS的平台，并使用多块数据分析策略成功处理了受试者间的高代谢变异性。强调了代谢物的特定子集与UGT 2B17酶的有影响力的遗传多态性之间的联系。结论这种探索性代谢组学策略是更好地了解导致类固醇代谢高度个体间变异性的潜在模式的第一步。选择有前景的生物标志物进行进一步的靶向研究。
AIM Antidoping procedures are expected to greatly benefit from untargeted metabolomic approaches through the discovery of new biomarkers of prohibited substances abuse. RESULTS Endogenous steroid metabolites were monitored in urine samples from a controlled elimination study of testosterone undecanoate after ingestion. A platform coupling ultra-high pressure LC with high-resolution quadrupole TOF MS was used and high between-subject metabolic variability was successfully handled using a multiblock data analysis strategy. Links between specific subsets of metabolites and influential genetic polymorphisms of the UGT2B17 enzyme were highlighted. CONCLUSION This exploratory metabolomic strategy constitutes a first step toward a better understanding of the underlying patterns driving the high interindividual variability of steroid metabolism. Promising biomarkers were selected for further targeted study.