Characterization of a sigma class GST(GSTS6)required for cellular detoxification and embryogenesis in Tribolium castaneum
Characterization of a sigma class GST(GSTS6)required for cellular detoxification and embryogenesis in Tribolium castaneum
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赤拟谷盗细胞解毒和胚胎发生所需的西格玛类 GST (GSTS6) 的表征
DOI：
10.1111/1744-7917.12930
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发表时间：
2022
期刊：
影响因子：
4
通讯作者：
Li Bin
中科院分区：
文献类型：
--
作者：
Song Xiaowen;Zhong Qisheng;Ji Yanhao;Zhang Yuemei;Tang Jing;Feng Fan;Bi Jingxiu;Xie Jia;Li Bin
sigma谷胱甘肽S转移酶（GSTs）是一类在昆虫抗氧化防御中起重要作用的胞质谷胱甘肽S转移酶（GSTs），但其参与抗氧化活性的机制尚不清楚。在此，我们分离了一种来自于castaneum的GSTS(GSTS6)，并对其功能进行了研究。同源性和系统发育分析表明，TcGSTS6与其他进化保守的gsts具有较高的同源性。重组TcGSTS6蛋白对异丙苯过氧化氢和4-羟基壬烯醛具有较强的活性，但对通用底物1-氯-2,4-二硝基苯的活性较低。暴露于各种类型的氧化应激，包括热、冷、紫外线和病原微生物，显著诱导TcGSTs6的表达，表明其参与抗氧化防御。通过RNA干扰（RNAi）敲低TcGSTs6，导致其抗氧化能力降低，这是通过与其他抗氧化基因协同完成的。此外，用硫辛硫磷、高效氯氰菊酯、敌敌畏和呋呋虫胺等多种杀虫剂处理后发现，TcGSTS6在杀虫剂解毒中起重要作用。RNAi结果表明，TcGSTS6基因在甘蔗胚发生中起重要作用。本研究阐明了GSTS对昆虫抗氧化活性的作用机制，加深了对GSTS功能多样性的认识。
The sigma glutathione S-transferases(GSTSs)are a class of cytosolic glutathione S transferases(GSTs)that play important roles in antioxidant defense in insects, but the mechanisms by which GSTSs contribute to antioxidant activity remain unclear. Here, we isolated a GSTS(GSTS6)from Tribolium castaneum and explored its function. Homology and phylogenetic analysis revealed that TcGSTS6 shared high identity with other evolutionarily conserved GSTSs. The recombinant TcGSTS6 protein had strong activity toward cumene hydroperoxide and 4-hydroxynonenal but low activity toward the universal substrate 1-chloro-2,4-dinitrobenzene. Exposure to various types of oxidative stress, including heat, cold, UV and pathogenic microbes, significantly induced TcGSTs6 expression, which indicates that it is involved in antioxidant defense. Knockdown TcGSTs6 by using RNA interference(RNAi)caused reduced antioxidant capacity, which was accomplished by cooperating with other antioxidant genes. Moreover, treatment with various insecticides such as phoxim, lambda-cyhalothrin, dichlorvos and carbofuran revealed that TcGSTS6 plays an important role in insecticide detoxification. The RNAi results showed that TcGSTS6 is essential for embryogenesis in T. castaneum. Our study elucidates the mechanism by which a GSTS contributes to antioxidant activity and enhances our understanding of the functional diversity of GSTSs in insects.