Fungal invasion of the rhizosphere microbiome
Fungal invasion of the rhizosphere microbiome
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DOI：
10.1038/ismej.2015.82
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发表时间：
2016-01-01
期刊：
影响因子：
11
通讯作者：
Raaijmakers, Jos M.
中科院分区：
文献类型：
--
作者：
Chapelle, Emilie;Mendes, Rodrigo;Raaijmakers, Jos M.
根际是土传病原体与植物建立寄生关系的感染场所。为了感染根组织，病原体必须与根际微生物组的成员竞争可用的营养物质和微位点。在抑病土壤中，病原菌的生长受到特定根际微生物的强烈限制。在这里，我们对生长在抑制真菌病原体立枯丝核菌的土壤中的甜菜幼苗根际微生物组的宏基因组DNA和RNA进行了测序。基于rRNA的分析表明，Oxalobacteraceae，Burkholderiaceae，Sphingobacteriaceae和Sphingomonadaceae在真菌入侵后根际中的含量显著增加。元转录组学显示，压力相关基因（ppGpp代谢和氧化应激）在这些细菌家族中上调。我们假设，入侵的病原真菌诱导，直接或通过植物，根际细菌群落的应激反应，导致微生物组组成的变化和激活的拮抗特性，限制病原体感染。
The rhizosphere is the infection court where soil-borne pathogens establish a parasitic relationship with the plant. To infect root tissue, pathogens have to compete with members of the rhizosphere microbiome for available nutrients and microsites. In disease-suppressive soils, pathogens are strongly restricted in growth by the activities of specific rhizosphere microorganisms. Here, we sequenced metagenomic DNA and RNA of the rhizosphere microbiome of sugar beet seedlings grown in a soil suppressive to the fungal pathogen Rhizoctonia solani. rRNA-based analyses showed that Oxalobacteraceae, Burkholderiaceae, Sphingobacteriaceae and Sphingomonadaceae were significantly more abundant in the rhizosphere upon fungal invasion. Metatranscriptomics revealed that stress-related genes (ppGpp metabolism and oxidative stress) were upregulated in these bacterial families. We postulate that the invading pathogenic fungus induces, directly or via the plant, stress responses in the rhizobacterial community that lead to shifts in microbiome composition and to activation of antagonistic traits that restrict pathogen infection.