Immunostimulatory human urinary protein.
Immunostimulatory human urinary protein.
复制标题
免疫刺激性人尿蛋白。
DOI：
10.1073/pnas.89.10.4358
复制
发表时间：
1992
影响因子：
11.1
通讯作者：
R. Fauve
中科院分区：
文献类型：
--
作者：
E. Fontan;H. Saklani;R. Fauve
We have previously extracted a protein from inflammatory mouse granuloma that fully protects normal mice against lethal Listeria monocytogenes infection. We now report that polyclonal antibodies directed against this protein react with a human urinary fraction that also provides full protection of normal mice against L. monocytogenes. Murine monoclonal antibodies completely inhibit the protective activity of the human urinary fraction. We have purified to apparent homogeneity a human glycoprotein of 43 kDa (HGP.43), pI = 3.2 +/- 0.2. HGP.43 injected i.v. at 250 micrograms/kg fully protected mice against a lethal inoculum of L. monocytogenes. Such resistance followed injection of HGP.43, 4 days before and, still significantly, 8 hr after Listeria infection. In scid mice lacking T and B cells, similar resistance to L. monocytogenes was observed. Inflammatory murine macrophages became cytostatic against Lewis carcinoma cells after incubation with 1.5 nM HGP-43.
