Prevalence and Diversity of Avian Influenza Virus Hemagglutinin Sero-Subtypes in Poultry and Wild Birds in Bangladesh
Prevalence and Diversity of Avian Influenza Virus Hemagglutinin Sero-Subtypes in Poultry and Wild Birds in Bangladesh
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DOI：
10.3390/vetsci7020073
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发表时间：
2020-06-01
影响因子：
2.4
通讯作者：
Hoque, Md. A.
中科院分区：
文献类型：
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作者：
Hassan, Mohammad M.;El Zowalaty, Mohamed E.;Hoque, Md. A.
高致病性禽流感H5病毒具有大流行的可能性，造成重大经济损失，是兽医和公共卫生问题。本研究旨在调查禽流感病毒(AIV)血凝素(HA)亚型在孟加拉国家禽和野生鸟类中的分布和多样性。我们在2012-2014年冬季对查托格拉姆和西尔赫特湿地的野生和家禽进行了禽流感血清监测。采用竞争性酶联免疫吸附试验(c-EL ISA)检测血清标本，随机抽取阳性血清标本170份，用血凝抑制法(HI)检测16种不同HA亚型的抗体。除H7、H11、H14和H15亚型外，其他亚型均以H5和H9为主，与禽种无关。群内HA亚型的多样性从3个(家禽)到10个(候鸟)。H5亚型在游牧鸭中的感染率为76.3%(29/38)，在家禽中为71.4%(5/7)，在留鸟中为66.7%(24/36)，在候鸟中为65.9%(27/41)，在家鸭中为61.7%(29/47)。H9亚型在候鸟中常见(56%；23/41)，其次是家鸭38.3%(18/47)、游牧鸭36.8%(14/38)、野鸟30.6%(11/66)和家鸡28.5%(2/7)。2012年候鸟中最常见的血清亚型是H1、H4和H6血清亚型(分别为80%；8/10，70%；7/10和70%；7/10)；2013年候鸟中最常见的血清亚型是H9(83.3%；5/6)，游鸭中最常见的血清亚型是H2(73.9%；17/23)，2014年所有鸟类中都有H5血清亚型(50%~100%)。本研究表明，在孟加拉国的不同鸟类中存在着高度多样性的HA亚型，这种广泛的AIV宿主可能增加AIV重新分类的可能性，并可能促进新的AIV毒株的出现。建议在目标家禽-野生鸟类交界处持续监测禽流感，以了解禽流感的生态和进化。
Highly pathogenic avian influenza H5 viruses have pandemic potential, cause significant economic losses and are of veterinary and public health concerns. This study aimed to investigate the distribution and diversity of hemagglutinin (HA) subtypes of avian influenza virus (AIV) in poultry and wild birds in Bangladesh. We conducted an avian influenza sero-surveillance in wild and domestic birds in wetlands of Chattogram and Sylhet in the winter seasons 2012-2014. We tested serum samples using a competitive enzyme-linked immunosorbent assay (c-ELISA), and randomly selected positive serum samples (170 of 942) were tested using hemagglutination inhibition (HI) to detect antibodies against the 16 different HA sero-subtypes. All AIV sero-subtypes except H7, H11, H14 and H15 were identified in the present study, with H5 and H9 dominating over other subtypes, regardless of the bird species. The diversity of HA sero-subtypes within groups ranged from 3 (in household chickens) to 10 (in migratory birds). The prevalence of the H5 sero-subtype was 76.3% (29/38) in nomadic ducks, 71.4% (5/7) in household chicken, 66.7% (24/36) in resident wild birds, 65.9% (27/41) in migratory birds and 61.7% (29/47) in household ducks. Moreover, the H9 sero-subtype was common in migratory birds (56%; 23/41), followed by 38.3% (18/47) in household ducks, 36.8% (14/38) in nomadic ducks, 30.6% (11/66) in resident wild birds and 28.5% (2/7) in household chickens. H1, H4 and H6 sero-subtypes were the most common sero-subtypes (80%; 8/10, 70%; 7/10 and 70%; 7/10, respectively) in migratory birds in 2012, H9 in resident wild birds (83.3%; 5/6) and H2 in nomadic ducks (73.9%; 17/23) in 2013, and the H5 sero-subtype in all types of birds (50% to 100%) in 2014. The present study demonstrates that a high diversity of HA subtypes circulated in diverse bird species in Bangladesh, and this broad range of AIV hosts may increase the probability of AIVs' reassortment and may enhance the emergence of novel AIV strains. A continued surveillance for AIV at targeted domestic-wild bird interfaces is recommended to understand the ecology and evolution of AIVs.