A YEAST PROTEIN PHOSPHATASE RELATED TO THE VACCINIA VIRUS VH1 PHOSPHATASE IS INDUCED BY NITROGEN STARVATION
A YEAST PROTEIN PHOSPHATASE RELATED TO THE VACCINIA VIRUS VH1 PHOSPHATASE IS INDUCED BY NITROGEN STARVATION
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DOI：
10.1073/pnas.89.24.12175
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发表时间：
1992-12-15
影响因子：
11.1
通讯作者：
DIXON, JE
中科院分区：
文献类型：
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作者：
GUAN, K;HAKES, DJ;DIXON, JE
与痘苗病毒 VH1 磷酸酶相关的磷酸酶已从酿酒酵母中克隆出来。酵母磷酸酶与粟酒裂殖酵母 cdc25 基因产物以及由哺乳动物开放阅读框 3CH134 编码的蛋白质有关，3CH134 是对血清刺激做出反应的早期基因。酵母基因产物的磷酸酶活性似乎仅限于水解含磷酸酪氨酸的底物，而牛痘磷酸酶水解含磷酸丝氨酸和含磷酸酪氨酸的底物。氮饥饿显着诱导编码酵母磷酸酶的 mRNA。酵母磷酸酶基因失活会导致生长速度下降。
A phosphatase related to the vaccinia virus VH1 phosphatase has been cloned from Saccharomyces cerevisiae. The yeast phosphatase is related to the Schizosaccharomyces pombe cdc25 gene product and to a protein encoded by a mammalian open reading frame known as 3CH134, which is an immediate early gene responding to serum stimulation. The phosphatase activity of the yeast gene product appears to be restricted to the hydrolysis of phosphotyrosine-containing substrates, whereas the vaccinia phosphatase hydrolyzes both phosphoserine- and phosphotyrosine-containing substrates. The mRNA encoding the yeast phosphatase is dramatically induced by nitrogen starvation. Inactivation of the yeast phosphatase gene results in a decrease in growth rate.