The transfer of touch DNA from hands to glass, fabric and wood
The transfer of touch DNA from hands to glass, fabric and wood
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DOI：
10.1016/j.fsigen.2010.12.016
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
McDermott, Sean D.
中科院分区：
文献类型：
--
作者：
Daly, Dyan J.;Murphy, Charlotte;McDermott, Sean D.
过去已经对通过握持或触摸将DNA从手上转移到物体上这一现象进行了研究。本研究的主要目的是检验从手转移到玻璃、织物和木材上的DNA量的差异。该研究涉及300名志愿者（100名用于玻璃，100名用于织物，100名用于木材），其中50%为男性，50%为女性。志愿者握持材料60秒。使用微型胶带提取法从物体上获取DNA，使用Quantifiler试剂盒进行定量，使用“Qiagen（R）QIAamp DNA微型试剂盒”进行提取，并使用AmpFlSTR（R）SGM Plus（TM）扩增试剂盒在28个循环下进行扩增。结果表明，通过方差分析，在回收的DNA量方面，三种物体类型之间存在显著差异（F = 8.2，p < 0.05）。就DNA转移和回收而言，木材的产量最高，其次是织物，然后是玻璃。从玻璃样本中获得能表明持有者的图谱的成功率约为9%，织物为23%，木材为36%。男性和女性志愿者转移的DNA量之间没有显著差异。在本研究中，根据获得6个或更多等位基因的有用图谱所定义的良好脱落者状态，估计约占人口的22%。当获得混合DNA图谱时观察到了二次转移现象，但发生率较低，约占样本总数的10%。在仅由一名志愿者触摸过的物体上发现了对应不止一个人的DNA图谱。尽管二次转移是可能的，但从被触摸物体上获得的图谱更有可能是一次转移的结果，而非二次来源。（C）2011爱思唯尔爱尔兰有限公司。保留所有权利。
The transfer of DNA from hands to objects by holding or touching has been examined in the past. The main purpose of this study was to examine the variation in the amount of DNA transferred from hands to glass, fabric and wood. The study involved 300 volunteers (100 for glass, 100 for fabric and 100 for wood) 50% of which were male and 50% female. The volunteers held the material for 60 s. The DNA was recovered from the objects using a minitape lift, quantified using the Quantifiler kit assay, extracted using a 'Qiagen (R) QIAamp DNA mini kit' and amplified using the AmpFlSTR (R) SGM Plus (TM) Amplification Kit at 28 cycles. The results show that using ANOVA there was a significant difference (F = 8.2, p < 0.05) between the three object types in the amount of DNA recovered. In terms of DNA transfer and recovery, wood gave the best yield, followed by fabric and then glass. The likelihood of success of obtaining a profile indicative of the holder was approximately 9% for glass samples, 23% for fabric and 36% for wood. There was no significant difference between the amount of DNA transferred by male or female volunteers. In this study good shedder status, as defined by obtaining useful profiles of 6 or more alleles, is estimated at approximately 22% of the population. The phenomenon of secondary transfer was observed when mixed DNA profiles were obtained but the incidence was low at approximately 10% of the total number of samples. DNA profiles corresponding to more than one person were found on objects which had been touched by only one volunteer. Although secondary transfer is possible the profiles obtained from touched objects are more likely to be as a result of primary transfer rather than a secondary source. (C) 2011 Elsevier Ireland Ltd. All rights reserved.