Spectroscopic and biological studies of spin-labeled tetracycline.
Spectroscopic and biological studies of spin-labeled tetracycline.
复制标题
自旋标记四环素的光谱和生物学研究。
DOI：
10.1016/0024-3205(92)90147-h
复制
发表时间：
1992
期刊：
影响因子：
6.1
通讯作者：
Pezeshk,V
中科院分区：
文献类型：
--
作者：
Pezeshk,A;Pezeshk,V
合成了一种新的硝酰基标记四环素。质子核磁共振实验四环素，自旋标记的四环素，并在DMSO-d 6的抗磁性减少形式的报告。在NMR谱中观察到的信号全部被指定。NMR数据显示，自旋标记物连接到四环素A环上的C-2酰胺基团上。自旋标记的四环素也在体外测试抗肿瘤活性，并发现对白血病P338/ADR细胞系和黑色素瘤LOX细胞系有活性。
A new nitroxyl labeled tetracycline is synthesized. Proton NMR experiments of tetracycline, spin-labeled tetracycline, and the diamagnetic reduced form in DMSO-d 6 are reported. The signals observed in the NMR spectra are all assigned. The NMR data revealed that the spin label is attached to the C-2 amide group on ring A of tetracycline. The spin-labeled tetracycline is also tested in vitro for antitumor activity and is found to be active against leukemia P338/ADR cell line and in melanoma LOX cell line.