Vectors for the expression of tagged proteins in Schizosaccharomyces pombe
Vectors for the expression of tagged proteins in Schizosaccharomyces pombe
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DOI：
10.1016/s0378-1119(98)00434-x
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发表时间：
1998-10-09
期刊：
影响因子：
3.5
通讯作者：
Carr, AM
中科院分区：
文献类型：
--
作者：
Craven, RA;Griffiths, DJF;Carr, AM
描述了一系列的载体，使蛋白质融合到不同的标签序列在pombe裂糖菌的episomal表达。蛋白质可以通过融合GFP/EGFP的氨基末端、HA或Pk表位的三个拷贝或包含myc表位的两个拷贝和六个组氨酸残基（MH）的组合标签来表达。GFP/EGFP或Pk可以将蛋白质的羧基末端与标签融合。融合蛋白的表达由可调节的nmt1启动子的中等强度突变版本控制。(C) 1998 Elsevier Science B.V.版权所有
A series of vectors is described which enables the episomal expression of proteins fused to different tag sequences in Schizosaccharomyces pombe. Proteins can be expressed with their amino termini fused to GFP/EGFP, three copies of the HA or Pk epitopes or a combined tag which contains two copies of the myc epitope and six histidine residues (MH). Fusion of the carboxyl terminus of a protein to a tag is possible with GFP/EGFP or Pk. Expression of the fusion proteins is controlled by the medium strength mutant version of the regulatable nmt1 promoter. (C) 1998 Elsevier Science B.V. All rights reserved.