Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs
Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs
复制标题
家蚕凋亡抑制剂 (IAP) 与核多角体病毒 IAP 之间相互作用的进化和功能分析
DOI：
10.1111/1744-7917.12664
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发表时间：
2020-06-01
期刊：
影响因子：
4
通讯作者：
Lu, Cheng
中科院分区：
文献类型：
--
作者：
Chen, Peng;Kang, Tao-Tao;Lu, Cheng
细胞凋亡作为昆虫的一种重要免疫反应，在杆状病毒与昆虫宿主相互作用中起着重要作用。以前的报道已经在昆虫和杆状病毒中鉴定出了凋亡抑制蛋白（IAP），但是这些蛋白之间的关系仍然不清楚。本研究中，我们发现昆虫IAP蛋白与杆状病毒IAP 3基因聚簇，表明杆状病毒iap3基因可能来源于鳞翅目或双翅目昆虫。我们证明家蚕细胞凋亡抑制因子（Bmiap）基因对家蚕细胞凋亡具有抑制作用。进一步分析Bmiap基因对B细胞增殖的影响。家蚕核型多角体病毒（BmNPV）感染家蚕细胞后，BmNPV iap和BmNPV iap基因均能促进BmNPV增殖。BmNPVIAP 1和IAP 2与BmIAP在家蚕细胞中直接相互作用，表明BmNPViap基因可能在BmNPV感染过程中被BmNPViap基因劫持。综上所述，我们的研究结果提供了重要的见解iap基因的功能关系，并提高我们的杆状病毒和昆虫宿主细胞凋亡的知识。
As an important insect immune response, apoptosis plays a critical role in the interaction between baculoviruses and insect hosts. Previous reports have identified inhibitor of apoptosis (IAP) proteins in both insects and baculoviruses, but the relationship between these proteins is still not clearly understood. Here, we found that insect IAP proteins were clustered with baculovirus IAP3, suggesting that the baculovirus iap3 gene might be derived from the Lepidoptera or Diptera. We demonstrated that Bombyx mori inhibitor of apoptosis (Bmiap) gene had an inhibitory effect on apoptosis in silkworm cells. Further analysis of the effects of Bmiap genes on the proliferation of B. mori nucleopolyhedrovirus (BmNPV) showed that both the Bmiap and BmNPV iap genes increased BmNPV proliferation after BmNPV infected silkworm cells. Our results also indicated that BmNPV IAP1 and IAP2 directly interacted with BmIAP in silkworm cells, implying that the Bmiap gene might be hijacked by BmNPV iap genes during BmNPV infection. Taken together, our results provide important insights into the functional relationships of iap genes, and improve our knowledge of apoptosis in baculoviruses and insect hosts.