EFFECT OF SAMPLING SITE AND COLLECTION METHOD ON VARIATIONS IN BASE-LINE CLINICAL PATHOLOGY PARAMETERS IN FISCHER-344 RATS .2. CLINICAL HEMATOLOGY
EFFECT OF SAMPLING SITE AND COLLECTION METHOD ON VARIATIONS IN BASE-LINE CLINICAL PATHOLOGY PARAMETERS IN FISCHER-344 RATS .2. CLINICAL HEMATOLOGY
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DOI：
10.1016/0272-0590(86)90115-6
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发表时间：
1986-11-01
期刊：
影响因子：
--
通讯作者：
IRONS, RD
中科院分区：
文献类型：
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作者：
SMITH, CN;NEPTUN, DA;IRONS, RD
采用同龄雄性Fischer-344大鼠，分析采血部位和采集方法对外周血液学参数的影响。采血部位为右心室、腹主动脉、腹腔静脉、眶后神经丛和尾巴。采集方法包括注射器(10ml)、真空容器(S)(3ml或3ml)。2)和毛细管。从尾巴采集的血液显示出白细胞计数大约是从其他部位采集的样本的两倍。与其他部位相比，从眶后神经丛和尾部采集的血液在白细胞计数、红细胞计数、血红蛋白和红细胞压积以及淋巴细胞和中性粒细胞的白细胞分类计数方面存在显著差异。从腹主动脉和第二个真空容器中采集的血液中，除尾血的血小板计数低于其他部位外，其余部位的红细胞、白细胞和血小板计数均低于其他部位。虽然参数值因取样点的不同而不同，但与所有其他方法相比，从右室血中获得的参数值变化最小，一致性最高。
An analysis of the influence of blood-sampling site and collection method on peripheral hematology parameters was conducted in age-matched male Fischer-344 rats. Sites examined for blood collection were the right ventricle, abdominal aorta, abdominal vena cava, retroorbital plexus, and tail. Collection methods used included syringe (10 ml), Vacutainer(s) (3 ml or 3 ml .times. 2), and capillary tube. Blood collected from the tail exhibited leukocyte counts approximately twice those of samples from other sites. Blood collected from the retroorbital plexus and tail exhibited significant variations in white blood cell count, red blood cell count, hemoglobin, and hematocrit, and differences in leukocyte differential counts of lymphocytes and neutrophils when compared with other sites. Blood collected from the abdominal aorta and in a second Vacutainer from the right ventricle exhibited lower erythyrocyte, leukocyte, and platelet counts than that collected from other sites with the exception of the platelet count from tail blood which was lower than that from all other sites. Although parameter values vary with sample site selection, those obtained from right ventricle blood were the least variable and the most consistent when compared with all other methods.