Large decreases in membrane phosphatidylethanolamine and diphosphatidylglycerol upon mutation to duramycin resistance do not change the protonophore resistance of Bacillus subtilis.
Large decreases in membrane phosphatidylethanolamine and diphosphatidylglycerol upon mutation to duramycin resistance do not change the protonophore resistance of Bacillus subtilis.
复制标题
耐久霉素抗性突变后膜磷脂酰乙醇胺和二磷脂酰甘油的大幅减少不会改变枯草芽孢杆菌的质子载体抗性。
DOI：
10.1016/0005-2736(88)90341-0
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Krulwich,TA
中科院分区：
文献类型：
--
作者：
DunkleyJr,EA;Clejan,S;Guffanti,AA;Krulwich,TA
从野生型枯草芽孢杆菌BD 99及其质子载体抗性突变株AG 1A 3中筛选出耐杜霉素突变株。膜的耐药性突变体的分析表明，他们有很少或没有磷脂酰乙醇胺和二磷脂酰甘油，通过化学检测后，薄层色谱法测定。少量的这些磷脂必须保留在突变株，但是，因为在外源性，放射性脂肪酸的掺入研究，与棕榈油酸相关的标签被发现在色谱位置，对应于预期的位置的磷脂酰乙醇胺和二磷脂酰甘油。耐度霉素菌株均显示磷脂酰甘油和氨酰（赖氨酰）磷脂酰甘油水平升高。质子载体抗性AG 1A 3的耐度霉素衍生物（AG 1A 3-DR 4），而不是野生型，也显示出相对于极性脂质的中性含量降低。该菌株的中性脂肪组成中游离脂肪酸含量较高，1，2-二酰基甘油含量较低。AG 1A 3-DR 4还含有明显水平的溶血磷脂酰乙醇胺和相对于研究中的其他菌株稍微升高的二糖基二酰基甘油。AG 1A 3的质子载体抗性不因突变而改变。外源棕榈油酸在降低AG 1A 3-DR 4的质子载体抗性方面的功效也没有任何变化。这种现象在磷脂酰乙醇胺和二磷脂酰甘油含量显著降低时持续存在，即使这些磷脂通常是介导该效应的外源性不饱和脂肪酸的优选掺入位点。
Duramycin-resistant mutant strains were selected from wild-typeBacillus subtilis(BD99) and its protonophore-resistant mutant derivative, strain AG1A3. Analyses of the membranes of the duramycin-resistant mutants showed that they had little or no phosphatidylethanolamine and diphosphatidylglycerol as determined by chemical detection after thin-layer chromatography. Small amounts of these phospholipids must remain in the mutant strains, however, because during studies of incorporation of exogenous, radioactive fatty acids, label associated with palmitoleic acid was found in chromatographic positions that corresponded to the expected positions of phosphatidylethanolamine and diphosphatidylglycerol. The duramycin-resistant strains both showed elevated levels of phosphatidylglycerol and aminoacyl(lysyl)phosphatidylglycerol. The duramycin-resistant derivative of protonophore-resistant AG1A3 (AG1A3-DR4), but not that of the wild type, also showed a decreased content of neutral relative to polar lipid in the membrane. The composition of neutral lipid in that strain was higher in free fatty acids and lower in 1,2-diacylglycerol than its parent strain. AG1A3-DR4 also contained appreciable levels of lysophosphatidylethanolamine and somewhat elevated diglycosyldiacylglycerol relative to the other strains in the study. The protonophore resistance of AG1A3 was unaltered by mutation to duramycin resistance. Nor was there any change in the efficacy of exogenous palmitoleic acid in diminishing the protonophore resistance of AG1A3-DR4. This phenomenon persists upon dramatic reduction in the content of phosphatidylethanolamine and diphosphatidylglycerol even though those phospholipids are normally the preferred sites of incorporation of the exogenous unsaturated fatty acids that mediate the effect.