Methods for in vitro characterization of multichannel nerve tubes
Methods for in vitro characterization of multichannel nerve tubes
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DOI：
10.1002/jbm.a.31298
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发表时间：
2008-03-01
影响因子：
4.9
通讯作者：
Windebank, Anthony J.
中科院分区：
文献类型：
--
作者：
de Ruiter, Godard C.;Onyeneho, Irene A.;Windebank, Anthony J.
多通道导管已被开发用于周围神经和脊髓的实验性修复。我们提出了一系列表征多通道神经管弯曲、变形、膨胀和降解特性的方法，并介绍了一种新的方法，通过不同大小的荧光葡聚糖分子（10、40和70 kDa）的扩散速率来测试多通道神经管的渗透性。首先，比较不同聚乳酸-羟基乙酸（PLGA）比例（50:50,75:25和85:15）制成的单腔神经管。一个比率（75:25 PLGA）随后用于比较单腔和多通道神经管。较低PLGA比例的神经管比较高PLGA比例的神经管更柔韧。然而，对于低比例，膨胀也更大，因为降解速度更快。多通道结构对管道的渗透率没有影响；在多通道75:25 PLGA神经管中的扩散速率似乎比在单腔神经管中的扩散速率更高，但这仅对70 kda分子有显著意义。此外，多通道75:25 PLGA神经管更灵活，同时更耐变形。然而，肿胀明显减少了总横截管腔面积，特别是在多通道75:25 PLGA神经管中。渗透性、弯曲、变形、肿胀和降解是多通道神经管发育的重要特征。本研究中提出的方法可以作为优化这些性质的基础，为将来可能的临床应用奠定基础。(C) 2007 Wiley期刊公司[J]生物材料研究。
Multichannel conduits have been developed for experimental peripheral nerve and spinal cord repair. We present a series of methods to characterize multichannel nerve tubes for properties of bending, deformation, swelling, and degradation and introduce a new method to test the permeability of multichannel nerve tubes from the rate of diffusion of different-sized fluorescent dextran molecules (10, 40, and 70 kDa). First, single-lumen nerve tubes made with different poly(lactic-co-glycolic acid) (PLGA) ratios (50:50, 75:25, and 85:15) were compared. One ratio (75:25 PLGA) was subsequently used to compare single-lumen and multichannel nerve tubes. Nerve tubes made with lower PLGA ratios were found to be more flexible than nerve tubes made with a higher PLGA ratio. For low ratios, however, swelling was also greater as a result of a faster rate of degradation. Multichannel structure did not interfere with the permeability of the tube; the rate of diffusion into multichannel 75:25 PLGA nerve tubes appeared to be even higher than that into single-lumen ones, but this was only significant for 70-kDa molecules. Also, multichannel 75:25 PLGA nerve tubes were more flexible and, at the same time, more resistant to deformation. However, swelling significantly decreased the total cross-sectional lumen area, especially in multichannel 75:25 PLGA nerve tubes. Permeability, bending, deformation, swelling, and degradation are important properties to characterize in the development of multichannel nerve tubes. The methods presented in this study can be used as a basis for optimizing these properties for future, possibly clinical, application. (C) 2007 Wiley Periodicals, Inc. J Biomed Mater Res.