Human keratinocyte culture. Identification and staging of epidermal cell subpopulations.
Human keratinocyte culture. Identification and staging of epidermal cell subpopulations.
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人类角质形成细胞培养物。
DOI：
10.1172/jci112317
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Hefton,JM
中科院分区：
文献类型：
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作者：
Staiano-Coico,L;Higgins,PJ;Darzynkiewicz,Z;Kimmel,M;Gottlieb,AB;Pagan-Charry,I;Madden,MR;Finkelstein,JL;Hefton,JM
在无真皮成分的培养系统中，研究了人表皮细胞分层成由基底层和基底上层（类似于表皮的生发层和棘层）组成的多层片。虽然没有角质层在体外发展，这种培养系统提供了一种方法来研究人类角质形成细胞分化的早期事件。吖啶橙染色的表皮细胞从这些文化的多参数流式细胞仪分析显示，三个不同的亚群不同的细胞大小，RNA含量和细胞周期动力学。第一个亚群由小的基底角质形成细胞组成，RNA含量低，世代时间长。第二个亚群由较大的角质形成细胞组成，具有较高的RNA含量和显着较短的世代时间。最后，第三个亚群含有最大的细胞，其不分裂，代表更终末分化的角质形成细胞。这种体外方法提供了有区别的细胞化学参数，通过这些参数可以在移植到人类烧伤患者身上之前评估表皮细胞片的成熟度。图片
Stratification of human epidermal cells into multilayered sheets composed of basal and suprabasal layers (resembling the stratum germinativum and stratum spinosum of the epidermis) was studied in a dermal component-free culture system. Although no stratum corneum developed in vitro, this culture system provided a method to study early events in human keratinocyte differentiation. Multiparameter flow cytometric analysis of acridine orange-stained epidermal cells from these cultures revealed three distinct subpopulations differing in cell size, RNA content, and cell cycle kinetics. The first subpopulation was composed of small basal keratinocytes with low RNA content and a long generation time. The second subpopulation consisted of larger keratinocytes, having higher RNA content and a significantly shorter generation time. Finally, the third subpopulation contained the largest cells, which did not divide, and represent the more terminally differentiated keratinocytes. This in vitro approach provides discriminating cytochemical parameters by which the maturity of the epidermal cell sheets can be assessed prior to grafting onto human burn patients.Images