Induction of prostate carcinoma in situ at high incidence in F344 rats by a combination of 3,2'-dimethyl-4-aminobiphenyl and ethinyl estradiol.
Induction of prostate carcinoma in situ at high incidence in F344 rats by a combination of 3,2'-dimethyl-4-aminobiphenyl and ethinyl estradiol.
复制标题
通过 3,2-二甲基-4-氨基联苯和乙炔雌二醇的组合在 F344 大鼠中诱导高发病率的原位前列腺癌。
作者：
T. Shirai;S. Fukushima;E. Ikawa;Y. Tagawa;N. Ito
雄性F344大鼠连续3周饲喂含0.75 ppm乙炔雌二醇的日粮，2周交替饲喂10次基础日粮，每次改为基础日粮后2天（饲喂基础日粮第3天），单次s.c注射3,2′-二甲基-4-氨基联苯50 mg/kg体重。饲喂基础日粮至第60周，处死后进行组织学检查。在此治疗后，21只大鼠中有18只（85.7%）发现前列腺癌，但在整个实验期间保持正常饮食但同时给予3,2'-二甲基-4-氨基联苯的19只大鼠中只有1只（5.2%）发现前列腺癌。所有的癌都是显微镜下的，并在前列腺的腹叶发展。这种方法可能最终导致一个有用的前列腺癌动物模型。
Male F344 rats were given a diet containing 0.75 ppm of ethinyl estradiol for 3 weeks and a basal diet for 2 weeks alternately 10 times, and 2 days after each change to basal diet (on the third day of feeding of the basal diet), a single s.c. injection of 50 mg/kg body weight of 3,2'-dimethyl-4-aminobiphenyl. Then they were given the basal diet until week 60 and sacrificed for histological examination. Prostatic carcinomas were found in 18 of 21 rats (85.7%) after this treatment but in only 1 of 19 rats (5.2%) maintained on normal diet throughout the experiment but given 3,2'-dimethyl-4-aminobiphenyl at the same times as the diet given to test rats. All the carcinomas were microscopic and developed in the ventral lobe of the prostate. This method may ultimately lead to a useful animal model of prostatic carcinoma.