BDNF reduces eEF2 phosphorylation and enhances novel protein synthesis in growth cones of dorsal root ganglia neurons.
BDNF reduces eEF2 phosphorylation and enhances novel protein synthesis in growth cones of dorsal root ganglia neurons.
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BDNF 降低 eEF2 磷酸化并增强背根神经节神经元生长锥中新型蛋白质的合成。
DOI：
10.1007/s11064-018-2541-8
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Cho Y and Takei N
中科院分区：
文献类型：
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作者：
Hoshi O;Sugizaki A;Cho Y and Takei N
在神经元生长锥中，由细胞外刺激物如导向分子调节的局部翻译为轴突发育提供了分子机制。在这项研究中，我们采用免疫细胞化学结合原子力显微镜研究核糖体蛋白在大鼠背根神经节（DRG）神经元生长锥的定位。核糖体蛋白P0/1/2和S6的免疫反应性，和新的蛋白质合成中观察到的中央，空间庞大的区域的生长锥。脑源性神经营养因子（BDNF）减少eEF 2磷酸化，表明其激活，并在30分钟内增强蛋白质合成。BDNF的作用被雷帕霉素完全抑制，雷帕霉素是一种哺乳动物雷帕霉素靶蛋白（mTOR）的抑制剂。这些结果表明，BDNF通过mTOR信号迅速激活DRG生长锥中的翻译并促进新蛋白的合成。
The local translation, which is regulated by extracellular stimuli such as guidance molecules, in growth cones of neurons provides a molecular mechanism for axonal development. In this study, we performed immunocytochemistry together with atomic force microscopy to investigate the localization of ribosomal proteins in the growth cones of rat dorsal root ganglion (DRG) neurons. The immunoreactivity of ribosomal protein P0/1/2 and S6, and novel protein synthesis were observed in the central, sterically bulky region of growth cones. Brain derived neurotrophic factor (BDNF) reduced the eEF2 phosphorylation, indicating its activation, and enhanced protein synthesis within 30 min. The effects of BDNF were completely inhibited by rapamycin, an inhibitor of mammalian target of rapamycin (mTOR). These results indicated that BDNF rapidly activates translation and enhances novel protein synthesis in growth cones of DRG though the mTOR signaling.