Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction.
Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction.
复制标题
铁限制过程中爱德华氏菌的差异蛋白质的差异蛋白质。
DOI：
10.1371/journal.pone.0132504
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Karsi A
中科院分区：
文献类型：
--
作者：
Dumpala PR;Peterson BC;Lawrence ML;Karsi A
鲶鱼爱德华氏菌是一种革兰氏阴性兼性厌氧细胞内细菌，可引起斑点叉尾鮰肠道败血症。铁是细菌必需的无机营养素，对于细菌的侵袭至关重要。宿主铁利用率的降低可能会对细菌病原体造成显着的应激，并被认为是导致毒力基因表达显着改变的信号。然而，铁限制对鲫鱼蛋白质丰度的确切影响尚不清楚。本研究的目的是鉴定体外铁限制条件下鲫鱼的差异丰度蛋白质。我们应用二维差异凝胶电泳 (2D-DIGE) 来确定差异丰富的蛋白质，并应用基质辅助激光解吸/电离飞行时间质谱 (MALDI TOF/TOF MS) 来鉴定蛋白质。还对差异丰度蛋白质进行了基因本体论和基于通路的功能建模。总共检测到 50 种独特的差异丰度蛋白质，由于铁限制，丰度差异至少为 2 倍 (p ≤ 0.05)。上调和下调蛋白的数量分别为37和13。我们注意到几种蛋白质，包括 EsrB、LamB、MalM、MalE、FdaA 和 TonB 依赖性血红素/血红蛋白受体家族蛋白对鲫鱼中的铁限制有反应。
Edwardsiella ictaluri is a Gram-negative facultative anaerobe intracellular bacterium that causes enteric septicemia in channel catfish. Iron is an essential inorganic nutrient of bacteria and is crucial for bacterial invasion. Reduced availability of iron by the host may cause significant stress for bacterial pathogens and is considered a signal that leads to significant alteration in virulence gene expression. However, the precise effect of iron-restriction on E. ictaluri protein abundance is unknown. The purpose of this study was to identify differentially abundant proteins of E. ictaluri during in vitro iron-restricted conditions. We applied two-dimensional difference in gel electrophoresis (2D-DIGE) for determining differentially abundant proteins and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF MS) for protein identification. Gene ontology and pathway-based functional modeling of differentially abundant proteins was also conducted. A total of 50 unique differentially abundant proteins at a minimum of 2-fold (p ≤ 0.05) difference in abundance due to iron-restriction were detected. The numbers of up- and down-regulated proteins were 37 and 13, respectively. We noted several proteins, including EsrB, LamB, MalM, MalE, FdaA, and TonB-dependent heme/hemoglobin receptor family proteins responded to iron restriction in E. ictaluri.