Studies on the allergenic significance and structure of rat serum albumin.
Studies on the allergenic significance and structure of rat serum albumin.
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大鼠血清白蛋白致敏意义及结构研究。
DOI：
10.4049/jimmunol.125.6.2544
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发表时间：
1980
影响因子：
4.4
通讯作者：
R. Siraganian
中科院分区：
文献类型：
--
作者：
U. Wahn;T. Peters;R. Siraganian
本研究评价了大鼠血清白蛋白及其蛋白水解片段激活人嗜碱性粒细胞IgE介导的组胺释放的能力。33例大鼠过敏患者中8例白细胞释放组胺与大鼠血清白蛋白。两个蛋白水解片段的大鼠血清白蛋白，每个构成的分子的一半，被用来研究IgE反应的抗原位点。这些片段与一些供体的细胞一起释放组胺，从而证明在完整大鼠血清白蛋白分子上总共至少4个位点的每个片段上存在至少2个抗原决定簇。然而，在2个片段中未回收大部分过敏原活性（总回收率平均值= 6.4%，范围在0.1 - 31%之间）。这种损失可能是由于大鼠白蛋白分子在第三结构域中间的切割，抗原位点丢失和/或由于与完整分子相比片段中的微小构象变化。当对来自不同患者的细胞进行测试时，片段中回收的活性百分比差异高达500倍。因此，分子上没有一个免疫显性位点对所有患者同等重要。所有8例患者的细胞也与小鼠血清白蛋白反应，但只有2例与牛血清白蛋白反应。小鼠和大鼠血清白蛋白上至少有1个决定簇与IgE交叉反应。
This study evaluated the capacity of rat serum albumin and of its proteolytic fragments to activate human basophils for IgE-mediated histamine release. The leukocytes from 8 out of 33 patients allergic to rats released histamine with rat serum albumin. Two proteolytic fragments of rat serum albumin, each constituting half of the molecule, were used to study the IgE-reactive antigenic sites. These fragments released histamine with the cells of some of the donors, thus demonstrating the presence of at least 2 antigenic determinants on each fragment for a total minimum of 4 sites on the intact rat serum albumin molecule. Most of the allergenic activity, however, was not recovered in the 2 fragments (total recovery mean = 6.4%, range between 0.1 and 31%). This loss could be due to cleavage of the rat albumin molecule in the middle of the third domain with loss of antigenic sites and/or due to minor conformational changes in the fragments as compared with the intact molecule. There was up to a 500-fold difference in the percent of activity recovered in the fragments when tested on cells from different patients. Therefore, there is no single immunodominant site on the molecule equally important for all patients. The cells of all 8 patients also reacted with mouse serum albumin but only 2 with bovine serum albumin. At least 1 determinant on mouse and rat serum albumin is cross-reactive with IgE.