Regions of microsynteny in Magnaporthe grisea and Neurospora crassa
Regions of microsynteny in Magnaporthe grisea and Neurospora crassa
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DOI：
10.1006/fgbi.2001.1286
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发表时间：
2001-07-01
影响因子：
3
通讯作者：
Woessner, JP
中科院分区：
文献类型：
--
作者：
Hamer, L;Pan, HQ;Woessner, JP
对一个含有稻瘟病菌（Magnaporthe grisea）110,467bp基因组DNA的细菌人工染色体（BAC）克隆进行了测序、注释，并与粗糙脉孢菌（Neurospora crassa）、白色念珠菌（Candida albicans）和酿酒酵母（Saccharomyces cerevisiae）的基因组进行了比较。在BAC序列中鉴定出26个开放阅读框（ORF），它们涉及多种生化途径。发现一个53kb的区域（包含26个ORF中的18个）与粗糙脉孢菌基因组的一部分存在同线性。存在完全共线性区域以及中断的共线性区域。与白色念珠菌或酿酒酵母没有明显的同线性。在两个在进化上已经分离了约2亿年的属中鉴定出包含高度保守基因的同线性区域，这引发了许多关于这些基因的功能和特性的生物学问题。（C）2001学术出版社
A bacterial artificial chromosome (BAC) clone containing 110,467 bp of genomic DNA from Magnaporthe grisea was sequenced, annotated, and compared to the genomes of Neurospora crassa, Candida albicans, and Saccharomyces cerevisiae. Twenty-six open reading frames (ORFs), involved in multiple biochemical pathways, were identified in the BAC sequence. A region of 53 kb, containing 18 of the 26 ORFs, was found to be syntenic to a portion of the N. crassa genome. Subregions of complete colinearity as well as interrupted colinearity were present. No synteny was evident with either C. albicans or S. cerevisiae. The identification of syntenic regions containing highly conserved genes across two genera that have been evolutionarily separated for similar to 200 million years elicits many biological questions as to the function and identity of these genes. (C) 2001 Academic Press.