Biocompatibility of microbially reduced graphene oxide in primary mouse embryonic fibroblast cells
Biocompatibility of microbially reduced graphene oxide in primary mouse embryonic fibroblast cells
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DOI：
10.1016/j.colsurfb.2012.12.036
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发表时间：
2013-05-01
影响因子：
5.8
通讯作者：
Kim, Jin-Hoi
中科院分区：
文献类型：
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作者：
Gurunathan, Sangiliyandi;Han, Jae Woong;Kim, Jin-Hoi
石墨烯纳米片是一种由sp（2）键合的碳原子组成的单原子厚的平面片状结构，它们以蜂窝状的晶格紧密排列，引起了理论研究和工业应用的极大关注。由于石墨烯基纳米材料具有潜在的应用价值，采用生物法从氧化石墨烯（GO）合成石墨烯是纳米技术领域的重要课题之一。提出了一种绿色、简单、无毒的以铜绿假单胞菌生物质为还原剂制备石墨烯的方法。使用一系列分析技术表征所得微生物还原的氧化石墨烯（M-rGO）。紫外-可见光谱证实了氧化石墨烯向石墨烯的转变。傅里叶变换红外光谱（FT-IR）被用来研究表面功能的变化。利用X射线衍射仪（XRD）和高分辨扫描电子显微镜（SEM）分别研究了石墨烯的结晶性质和形貌。此外，使用原代小鼠胚胎成纤维细胞（PMEF）研究了M-rGO的生物相容性。目前的研究表明，M-rGO对PMEF细胞具有显著的生物相容性，即使在100 μ g/ml的高浓度下。因此，所提出的安全和绿色方法赋予M-rGO用于各种生物医学应用的巨大潜力。(C)2013爱思唯尔有限公司版权所有。
Graphene nanosheet is a one-atom thick planar sheet of sp(2)-bonded carbon atoms, which are densely packed in a honeycomb crystal lattice, attracting tremendous attention from both fundamental research and industrial applications. The synthesis of graphene from graphene oxide (GO) using a biological method is one of the important topics in the areas of nanotechnology, because graphene-based nanomaterials have potential applications. A green, simple and non-toxic method for preparing graphene using biomass of Pseudomonas aeruginosa as the reducing reagent is proposed. The resulting microbially reduced graphene oxide (M-rGO) was characterized using a range of analytical techniques. UV-visible spectroscopy confirms the transition of graphene oxide to graphene. Fourier transform infrared spectroscopy (FT-IR) was used to study the changes in surface functionalities. X-ray diffraction (XRD) and high resolution scanning electron microscopy (SEM) were used to investigate the crystalline nature and the morphologies of prepared graphene respectively. Furthermore, the biocompatibility of the M-rGO was investigated using primary mouse embryonic fibroblast (PMEF) cells. The present study suggests that the M-rGO has significant biocompatibility for PMEF cells, even at a high concentration of 100 mu g ml(-1). Therefore, the proposed safe and green method confers the M-rGO with a great potential for various biomedical applications. (C) 2013 Elsevier B.V. All rights reserved.