Thymosin fraction 5 (TF5) stimulates secretion of adrenocorticotropic hormone (ACTH) from cultured rat pituitaries.
Thymosin fraction 5 (TF5) stimulates secretion of adrenocorticotropic hormone (ACTH) from cultured rat pituitaries.
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胸腺肽 5 (TF5) 刺激培养的大鼠垂体分泌促肾上腺皮质激素 (ACTH)。
DOI：
10.1016/0024-3205(88)90378-5
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发表时间：
1988
期刊：
影响因子：
6.1
通讯作者：
Goldstein,AL
中科院分区：
文献类型：
--
作者：
McGillis,JP;Hall,NR;Goldstein,AL
在体内给药的部分纯化的胸腺提取物，TF 5，胸腺，含胸腺肽，引起血清糖皮质激素的增加。TF 5对肾上腺皮质酮分泌没有直接作用，这表明它是在垂体水平介导的。培养的大鼠垂体单层细胞用于确定是否通过刺激垂体分泌ACTH介导该效应。两个批次的TF 5（BPP 100和C114080-01）引起培养的垂体单层分泌ACTH呈剂量依赖性。有一个协同效应时，细胞与TF 5和促肾上腺皮质激素释放因子（CRF）处理。进行免疫中和研究，其中用TF 5或CRF和CRF抗体处理细胞。该抗体完全阻断CRF诱导的ACTH释放，但对TF 5刺激的ACTH释放没有影响，表明该活性不是由于TF 5中的CRF样肽。评价了从TF 5分离的许多肽和由免疫系统产生的某些其它肽刺激ACTH分泌的能力。这些包括胸腺素（TSN）α1、α11和β4、前胸腺素α（PT α）、胸腺生成素5（TP 5）、胸腺系列因子（FTS）、干扰素α（INF α）、INF γ、白细胞介素1（IL-1）和白细胞介素2（IL-2）。这些因素对垂体ACTH分泌无任何影响。这些结果表明，TF 5的某些肽组分通过刺激垂体ACTH释放引起血清皮质类固醇增加。
In vivo administration of a partially purified thymic hormone-containing extract of the thymus gland, TF5, causes an increase in serum glucocorticoids. The lack of a direct effect of TF5 on adrenal corticosterone secretion suggests that it is mediated at the level of the pituitary. Cultured rat pituitary monolayers were used to determine if the effect is mediated by stimulation of ACTH secretion from the pituitary. Two lots of TF5, BPP100 and C114080-01, caused a dose dependent secretion of ACTH from cultured pituitary monolayers. There was a synergistic effect when the cells were treated with both TF5 and corticotropin-releasing factor (CRF). Immunoneutralization studies were done in which the cells were treated with TF5 or CRF and an antibody to CRF. The antibody completely blocked CRF induced ACTH release, but had no effect on TF5 stimulated ACTH release, suggesting that the activity is not due to a CRF-like peptide in TF5. A number of peptides isolated from TF5, and certain other peptides produced by the immune system were evaluated for their ability to stimulate ACTH secretion. These included thymosin (TSN) α1, α11, and β4, prothymosin α (PT α, thymopoeitin 5 (TP5), factuer thymique serique (FTS), interferon α (INF α), INF γ, interleukin 1 (IL-1), and interleukin 2 (IL-2). None of these factors had any effect on pituitary ACTH secretion. These results demonstrate that some peptide component of TF5 causes an increase in serum corticosteroids by stimulating pituitary ACTH release.