Fucoxanthin and its metabolite, fucoxanthinol, suppress adipocyte differentiation in 3T3-L1 cells.
Fucoxanthin and its metabolite, fucoxanthinol, suppress adipocyte differentiation in 3T3-L1 cells.
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DOI：
10.3892/ijmm.18.1.147
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发表时间：
2006-07
影响因子：
5.4
通讯作者：
Hayato Maeda;M. Hosokawa;Tokutake Sashima;N. Takahashi;T. Kawada;K. Miyashita
中科院分区：
文献类型：
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作者：
Hayato Maeda;M. Hosokawa;Tokutake Sashima;N. Takahashi;T. Kawada;K. Miyashita
岩藻黄质是一种主要的类胡萝卜素，存在于食用海藻中，例如裙带菜和羊栖菜。我们研究了岩藻黄质及其代谢物岩藻黄质醇对 3T3-L1 前脂肪细胞向脂肪细胞分化的抑制作用。岩藻黄质抑制 3T3-L1 细胞脂肪细胞分化过程中细胞间脂质积累。此外，岩藻黄质在 3T3-L1 细胞中转化为岩藻黄质醇。岩藻黄醇还表现出对脂质积累的抑制作用，并降低甘油-3-磷酸脱氢酶活性（脂肪细胞分化的指标）。岩藻黄质醇的抑制作用强于岩藻黄质。此外，在用岩藻黄质和岩藻黄质醇处理的3T3-L1细胞中，调节脂肪形成基因表达的过氧化物酶体增殖物激活受体γ（PPARγ）以剂量依赖性方式下调。这些结果表明岩藻黄质和岩藻黄质醇通过下调PPARγ抑制3T3-L1细胞的脂肪细胞分化。岩藻黄质对3T3-L1细胞脂肪细胞分化的抑制作用比岩藻黄质更强。
Fucoxanthin is a major carotenoid found in edible seaweed such as Undaria pinnatifida and Hijikia fusiformis. We investigated the suppressive effects of fucoxanthin and its metabolite, fucoxanthinol, on the differentiation of 3T3-L1 preadipocytes to adipocytes. Fucoxanthin inhibited intercellular lipid accumulation during adipocyte differentiation of 3T3-L1 cells. Furthermore, fucoxanthin was converted to fucoxanthinol in 3T3-L1 cells. Fucoxanthinol also exhibited suppressive effects on lipid accumulation and decreased glycerol-3-phosphate dehydrogenase activity, an indicator of adipocyte differentiation. The suppressive effect of fucoxanthinol was stronger than that of fucoxanthin. In addition, in 3T3-L1 cells treated with fucoxanthin and fucoxanthinol, peroxisome proliferator-activated receptor gamma (PPARgamma), which regulates adipogenic gene expression, was down-regulated in a dose-dependent manner. These results suggest that fucoxanthin and fucoxanthinol inhibit the adipocyte differentiation of 3T3-L1 cells through down-regulation of PPARgamma. Fucoxanthinol had stronger suppressive effects than fucoxanthin on adipocyte differentiation in 3T3-L1 cells.