Developed and evaluated a multiplex mRNA profiling system for body fluid identification in Chinese Han population.
Developed and evaluated a multiplex mRNA profiling system for body fluid identification in Chinese Han population.
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DOI：
10.1016/j.jflm.2015.08.006
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发表时间：
2015-10
影响因子：
1.5
通讯作者：
F. Song;Haibo Luo;Yiping Hou
中科院分区：
文献类型：
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作者：
F. Song;Haibo Luo;Yiping Hou
在法医案件工作中，从生物样本中识别细胞来源对于案件调查和犯罪现场重建至关重要。DNA/RNA共提取用于STR分型和体液鉴定已被认为是一种有效和全面的法医分析方法。以前的研究已经提出了几种细胞特异性信使RNA(信使RNA)标记来识别体液。本研究利用逆转录终点聚合酶链式反应(RT-PCR)技术，建立了一套包含19个标记的多重基因表达谱分析系统。该复合体结合了3个看家基因标记物和16个细胞特异性标记物，已被用于识别五种类型的人体体液：外周血液、精液、唾液、阴道分泌物和月经血。本研究对该混合物的专属性、灵敏度、稳定性和可检出性进行了探讨。fi。大多数细胞特异性fi标记显示高度特异的城市，尽管偶尔也观察到交叉反应。获得了每种体液的特异性图谱。此外，根据A.Lindenbergh等人的解释指南进行了体液类型的推断。评分指南可以应用于任何基于六种不同评分类别的RNA复合体(观察、观察和配合、零星观察和配合、未观察、零星观察、不可靠以及由于高输入而非特异性)。同时提取的DNA显示所有样品的全部或部分图谱结果为阳性。结果表明，STR图谱和RNA图谱相结合的方法用于中国汉族人群体液来源和供体的检测是可行和可靠的。
In forensic casework, identification the cellular origin from a biological sample is crucial to the case investigation and reconstruction in crime scene. DNA/RNA co-extraction for STR typing and human body fluids identification has been proposed as an efficient and comprehensive assay for forensic analysis. Several cell-specific messenger RNA (mRNA) markers for identification of the body fluids have been proposed by previous studies. In this study, a novel multiplex mRNA profiling system included 19 markers was developed and performed by reverse transcription endpoint polymerase chain reaction (RT-PCR). The multiplex combined 3 housekeeping gene markers and 16 cell-specific markers that have been used to identify five types of human body fluids: peripheral blood, semen, saliva, vaginal secretions and menstrual blood. The specificity, sensitivity, stability and detectability of the mixture were explored in our study. Majority of the cell-specific mRNA markers showed high specificity, although cross-reactivity was observed sporadically. Specific profiling for per body fluid was obtained. Moreover, the interpretation guidelines for inference of body fluid types were performed according to the A. Lindenbergh et al. The scoring guidelines can be applied to any RNA multiplex, which was based on six different scoring categories (observed, observed and fits, sporadically observed and fits, not observed, sporadically observed, not reliable, and non-specific due to high input). The simultaneous extraction of DNA showed positive full or partial profiling results of all samples. It demonstrated that the approach of combined STR-profiling and RNA profiling was suitable and reliable to detect the donor and origin of human body fluids in Chinese Han population.