Involvement of cross-reactive carbohydrate determinants-specific IgE in pollen allergy testing.
Involvement of cross-reactive carbohydrate determinants-specific IgE in pollen allergy testing.
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DOI：
10.5415/apallergy.2017.7.1.29
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发表时间：
2017-01
影响因子：
1.7
通讯作者：
Saito K
中科院分区：
文献类型：
--
作者：
Yokoi H;Yoshitake H;Matsumoto Y;Kawada M;Takato Y;Shinagawa K;Sakurai H;Saito K
针对不桥接肥大细胞上IgE分子的低分子碳水化合物抗原的特异性IgE抗体与临床症状无关。当花粉过敏原和植物源性食物过敏原之间的碳水化合物结构相似时，可以在过敏原特异性IgE检测试验中确定交叉反应性；在这种情况下，对谷物或豆类过敏原不敏感的花粉过敏患者可以报告假阳性结果。这种现象的产生是由于交叉反应性碳水化合物决定因子（CCDs）的存在。本研究旨在评估CCD干扰对普通成人花粉过敏原特异性IgE抗体结果的影响，并进行CCD抑制试验，评估CCD对花粉过敏原阳性样本的影响。对322名受试者的血清样本进行了花粉和CCD的IgE抗体检测。研究对象收到了关于花粉过敏症状的问卷，以帮助评估过敏的存在。检测雪松、柏树、果园草、豚草、MUXF、菠萝蛋白酶、辣根过氧化物酶（HRP）和抗坏血酸氧化酶（ASOD）的过敏原IgE抗体。结果显示，在花粉过敏原检测阳性的人群中，HRP的ccd特异性IgE抗体阳性率最高（13.5% ~ 50.0%）。抑制试验结果显示，CCD是轻微存在的。虽然针对雪松花粉的IgE抗体与CCD不发生反应，但针对日本柏树、果园草和豚草的IgE抗体可以通过CCD检测到。抑制试验结果显示明显的CCD存在，提示其参与。考虑到这些发现，应对日本柏树、果园草和豚草的患者IgE结果进行仔细评估。
Specific IgE antibodies against the low-molecular-weight carbohydrate antigen that does not bridge IgE molecules on mast cells are not associated with clinical symptoms. Cross reactivity can be determined in allergen-specific IgE detection assays when the carbohydrate structures between pollen allergens and plant derived food allergens are similar; in such cases, false positive results for grain or legume allergens can be reported for pollen allergic patients who are not sensitized to those allergens. This phenomenon arises owing to the presence of cross-reactive carbohydrate determinants (CCDs). This study aimed to assess the impact of CCD interference on the results for pollen allergen-specific IgE antibodies in the general adult population and to perform CCD inhibition tests evaluating the involvement of CCD on samples positive to pollen allergens. Serum samples from 322 subjects were tested for IgE antibodies to pollens and CCD. The research subjects were given questionnaires about pollen allergic symptoms to help assess the presence of allergies. Allergen IgE antibodies for Japanese cedar, Japanese cypress, orchard grass, ragweed, MUXF, bromelain, horseradish peroxidase (HRP), and ascorbate oxidase (ASOD) were analyzed. It was observed that among individuals who tested positive to any of the pollen allergens, the positive ratio of CCD-specific IgE antibody was the highest for HRP (13.5%–50.0%). The results from the inhibition tests revealed that CCD was marginally present. Although IgE antibodies for cedar pollen did not react with CCD, IgE antibodies for Japanese cypress, orchard grass, and ragweed might be detected by the presence of CCD. The results of the inhibition tests revealed the obvious presence of CCD suggesting its involvement. Considering these findings, careful evaluation of patient IgE results should be performed for Japanese cypress, orchard grass, and ragweed.