THE HUMAN ISLET AMYLOID POLYPEPTIDE (IAPP) GENE - ORGANIZATION, CHROMOSOMAL LOCALIZATION AND FUNCTIONAL IDENTIFICATION OF A PROMOTER REGION
THE HUMAN ISLET AMYLOID POLYPEPTIDE (IAPP) GENE - ORGANIZATION, CHROMOSOMAL LOCALIZATION AND FUNCTIONAL IDENTIFICATION OF A PROMOTER REGION
复制标题
DOI：
10.1016/0014-5793(90)80314-9
复制
发表时间：
1990-07-02
期刊：
影响因子：
3.5
通讯作者：
BETSHOLTZ, C
中科院分区：
文献类型：
--
作者：
CHRISTMANSON, L;RORSMAN, F;BETSHOLTZ, C
我们报道了编码胰岛淀粉样蛋白多肽（IAPP）的人类基因的分离和鉴定。先前表征的cDNA序列对应于三个外显子，其中第一个是非编码的。在5'侧DNA上鉴定出一个功能性启动子区域；然而，这比预期的要上游得多。对人胰岛素瘤RNA进行Northern blot分析，发现三个大小分别为1.2、1.8和2.1 kb的IAPP mrna，与该基因3'端存在的三个多聚腺苷化信号一致。对中期染色体进行原位杂交，在12号染色体12p12-p13和12q13-q14处有两个明显的峰。基因组DNA的Southern blot分析表明只有一个IAPP位点，但也表明在人类基因组DNA中存在其他同源序列。
We report the isolation and characterization of the human gene encoding islet amyloid polypeptide (IAPP). Previously characterized cDNA sequences correspond to three exons of which the first is noncoding. A functional promoter region was identified in the 5' flanking DNA; however, this was farther upstream than expected. Northern blot analysis of human insulinoma RNA revealed three IAPP mRNAs of sizes 1.2, 1.8 and 2.1 kb, in agreement with three polyadenylation signals present in the 3' end of the gene. In situ hybridization to metaphase chromosomes resulted in two distinct peaks on chromosome 12, at 12p12-p13 and 12q13-q14. Southern blot analysis of genomic DNA suggested a single IAPP locus but also indicated the presence of additional homologous sequences in human genomic DNA.