The effect of Ginkgo biloba extract on scopolamine-induced apoptosis in the hippocampus of rats
The effect of Ginkgo biloba extract on scopolamine-induced apoptosis in the hippocampus of rats
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DOI：
10.1007/s12565-013-0188-8
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发表时间：
2013-09-01
影响因子：
1.2
通讯作者：
Babakordi, F.
中科院分区：
文献类型：
--
作者：
Jahanshahi, M.;Nickmahzar, E. G.;Babakordi, F.
细胞凋亡，又称程序性细胞死亡，在正常发育和组织内稳态中起着至关重要的作用。细胞凋亡也与阿尔茨海默病等神经退行性疾病有关。健忘症指的是记忆力丧失，也可能是神经退行性疾病的警告信号。银杏叶提取物的抗氧化性已为人所知。因此，本研究旨在观察银杏叶提取物对东莨菪碱所致记忆障碍大鼠海马神经细胞凋亡数的影响。选用成年雄性Wistar大鼠36只。将大鼠随机分为对照组、假手术组、保护组和治疗组。假手术组大鼠仅给予氢溴酸东莨菪碱(3 mg/kg)腹腔注射。保护组和治疗组大鼠在注射东莨菪碱前/后给予银杏叶提取物(40，80 mg/kg)，连续7d。最后一次注射后48h取脑，用多聚甲醛固定，经组织学处理后，用TUNEL试剂盒染色检测神经细胞凋亡。数据通过单因素方差检验进行比较；P<；0.05被认为具有统计学意义。结果表明，与对照组相比，假手术组大鼠海马区各区神经元的凋亡数明显增加。而银杏叶提取物可减少东莨菪碱作用前后大鼠海马神经细胞的凋亡。结论：银杏叶提取物对大鼠海马神经细胞有保护作用，并可限制海马区的细胞凋亡。
Apoptosis, known as programmed cell death, plays a crucial role in normal development and tissue homeostasis. Apoptosis is also involved in neurodegenerative diseases such as Alzheimer's disease. Amnesia refers to the loss of memory and can also be a warning sign of neurodegenerative diseases. The antioxidant properties of Ginkgo biloba extract was known previously. Therefore, the aim of this study was to examine the effects of Ginkgo biloba extract on the rat's hippocampal apoptotic neurons number after Scopolamine based amnesia. Thirty-six adult male Wistar rats were used. Rats were randomly divided into control, sham, protective and treatment groups. The rats in the sham group received only scopolamine hydrobromide (3 mg/kg) intraperitoneally. The rats in the protective and treatment groups received Ginkgo biloba extract (40, 80 mg/kg) for 7 days intraperitoneally before/after scopolamine injection. Then 48 h after the last injection, the brains of rats were withdrawn and fixed with paraformaldehyde, and then, after histological processing, the slices were stained with the TUNEL kit for apoptotic neurons. Data were compared by the ANOVA Post Hoc Tukey test; P < 0.05 was considered significant. Our results showed that Scopolamine (in the sham group) increased significantly the number of apoptotic neurons in all areas of the hippocampus compared with the control. Whereas, Ginkgo biloba extract reduce the neuronal apoptosis in the hippocampus before and/or after encounter with scopolamine. We concluded that pretreatment and treatment injection of Ginkgo biloba extract can have a protective effect for neurons and it can limit apoptosis in all area of the hippocampus.