Dietary Omega-3 Fatty Acid Supplementation Reduces Inflammation in Obese Pregnant Women: A Randomized Double-Blind Controlled Clinical Trial.
Dietary Omega-3 Fatty Acid Supplementation Reduces Inflammation in Obese Pregnant Women: A Randomized Double-Blind Controlled Clinical Trial.
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DOI：
10.1371/journal.pone.0137309
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hauguel-de Mouzon S
中科院分区：
文献类型：
--
作者：
Haghiac M;Yang XH;Presley L;Smith S;Dettelback S;Minium J;Belury MA;Catalano PM;Hauguel-de Mouzon S
长链 omega 3 脂肪酸、二十碳五烯酸（EPA，20:5n-3）和二十二碳六烯酸（DHA，22:6n-3）对人体具有有效的抗炎特性。本研究表征了 omega-3 ω-3 脂肪酸补充剂 (ω-3 FA) 对超重/肥胖孕妇胎盘和脂肪组织炎症状态的影响。在超重/肥胖孕妇中进行了一项随机、双盲对照试验，这些孕妇从第 10-16 周到足月，被随机分配接受 DHA 加 EPA（2 克/天）或相当于安慰剂的治疗，每天两次。炎症途径的特征在于：1）治疗与未治疗女性的脂肪组织和胎盘； 2) 用长链 FA 培养的脂肪和滋养层细胞。与未治疗的女性相比，接受治疗的女性血浆 DHA 和 EPA 的总和增加了 5.8 倍，并且 ω-3 FA/ ω-6 FA 比率为 1.5 (p< 0.005)。血浆 CRP 浓度降低 (p<0.001)。接受治疗的女性的脂肪组织和胎盘表现出 TLR4 脂肪和胎盘表达以及 IL6、IL8 和 TNFα 的显着降低。在体外，EPA 和 DHA 抑制了脂肪和滋养层细胞培养物中棕榈酸酯诱导的 TLR4、IL6、IL8 的激活。超重/肥胖孕妇在膳食中补充 ω-3 FA 超过 25 周可减少母体脂肪和胎盘组织的炎症。 TLR4 似乎是细胞水平抗炎作用的中心靶标。临床试验.gov NCT00957476
Long-chain omega 3 fatty acids, eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) exert potent anti-inflammatory properties in humans. This study characterized the effects of omega-3 ω-3 fatty acids supplements (ω-3 FA) on the inflammatory status in the placenta and adipose tissue of overweight/obese pregnant women. A randomized, double-masked controlled trial was conducted in overweight/obese pregnant women that were randomly assigned to receive DHA plus EPA (2g/day) or the equivalent of a placebo twice a day from week 10–16 to term. Inflammatory pathways were characterized in: 1) adipose tissue and placenta of treated vs. untreated women; and 2) adipose and trophoblast cells cultured with long chain FAs. The sum of plasma DHA and EPA increased by 5.8 fold and ω-3 FA/ ω-6 FA ratio was 1.5 in treated vs. untreated women (p< 0.005). Plasma CRP concentrations were reduced (p<0.001). The adipose tissue and placenta of treated women exhibited a significant decrease in TLR4 adipose and placental expression as well as IL6, IL8, and TNFα In vitro, EPA and DHA suppressed the activation of TLR4, IL6, IL8 induced by palmitate in culture of adipose and trophoblast cells. Supplementation of overweight/obese pregnant women with dietary ω-3 FAs for >25 weeks reduced inflammation in maternal adipose and the placental tissue. TLR4 appears as a central target of the anti-inflammatory effects at the cellular level. ClinicalTrials.gov NCT00957476