The Topography of Microstructured Surfaces Differently Affects Fibrillin Deposition by Blood and Lymphatic Endothelial Cells in Culture
The Topography of Microstructured Surfaces Differently Affects Fibrillin Deposition by Blood and Lymphatic Endothelial Cells in Culture
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DOI：
10.1089/ten.tea.2007.0421
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发表时间：
2009-03-01
影响因子：
4.1
通讯作者：
Weber, Elisabetta
中科院分区：
文献类型：
--
作者：
Rossi, Antonella;Pasqui, Daniela;Weber, Elisabetta
虽然组织工程血管已经成功地应用于外科实践，但在需要时人工恢复淋巴循环仍远未实现。动脉血管壁的稳定性取决于适当的纤维蛋白沉积；纤维蛋白实际上是弹性纤维形成的支架。在淋巴管中，原纤维蛋白可能隐含在淋巴形成中，以响应间质需求。本研究旨在验证纤维蛋白沉积是否受到血液和淋巴内皮细胞生长的底物地形的影响。血液和淋巴内皮细胞在不同形貌的微结构表面上培养：不同宽度的条纹（25,50和100mm），正方形和长方形，螺旋几何形状，通过光固定透明质酸（Hyal）在氨基硅化玻璃上获得。细胞取向和纤维蛋白沉积受微观结构形貌的影响。血液内皮细胞沉积的纤维蛋白呈束状，平行于条纹和螺旋的主轴，而淋巴内皮细胞沉积的不规则纤维蛋白网络仅在最小的条纹中受底物地形的影响。这些数据为设计能够适应周围环境功能要求的组织工程血液和淋巴管所需的基本知识做出了贡献。
While tissue-engineered blood vessels have already been successfully used in surgical practice, artificially restoring lymphatic circulation when needed is still far to be realized. Stability of arterial vessel wall depends on proper fibrillin deposition; fibrillin in fact is the scaffold for elastic fiber formation. In lymphatic vessels fibrillin is probably implied in lymph formation in response to interstitial requirements. This study was designed to verify whether fibrillin deposition is influenced by the topography of the substrate on which blood and lymphatic endothelial cells grow. Blood and lymphatic endothelial cells were cultured on microstructured surfaces with different topography: stripes of different widths (25, 50, and 100 mm), squares and rectangles, and spiral geometry, obtained by the photoimmobilization of Hyaluronan (Hyal) on aminosilanized glass. Cell orientation and fibrillin deposition were influenced by the topography of the microstructure. Blood endothelial cells deposited fibrillin as a bundle running parallel to the major axis of stripes and spirals, whereas the irregular network of fibrillin deposited by lymphatic endothelial cells was affected by the topography of the substrate only in the smallest stripes. These data bring a contribution to the basic knowledge required to design tissue-engineered blood and lymphatic vessels capable of adapting to the functional requirements of the surrounding environment.