Using GC-MS for the quick analysis of carbon dioxide for soil microbial biomass determination
Using GC-MS for the quick analysis of carbon dioxide for soil microbial biomass determination
复制标题
使用 GC-MS 快速分析二氧化碳以测定土壤微生物量
DOI：
10.1039/c1ay05316g
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发表时间：
2011
影响因子：
3.1
通讯作者：
David J. Bailey
中科院分区：
文献类型：
--
作者：
B. Koerner;W. Calvert;David J. Bailey
微生物量碳是用来评价土壤环境中微生物丰度的常用参数，微生物量会随着干扰和/或环境条件的变化而变化。采用氯仿熏蒸-培养法对192个土壤样品进行微生物量碳测定，10d后采集气体样品。对气体样本进行了二氧化碳(CO2)浓度分析。二氧化碳浓度的测定通常是通过红外气体分析或化学分析(碱捕集和随后的滴定，或钠钙吸收)进行的，对于分析的样品数量来说，这些方法要么不可用，要么太耗时。因此，我们开发了一种用气相色谱/质谱仪(GC-MS)测定气体中二氧化碳的分析方法。该方法需要5μ的L注射，5分钟的分析时间，所得的优值系数可与文献中的大多数程序相媲美。此外，获得的微生物生物量碳值与以前在类似生态系统中公布的值一致。
Microbial biomass carbon is a common soil parameter used to evaluate microbial abundance in soil environments, and microbial biomass can change in response to disturbances and/or changing environmental conditions. Microbial biomass carbon was determined on 192 soil samples using the chloroform fumigation-incubation method, and after 10 days, gas samples were collected. Gas samples were analyzed for carbon dioxide (CO2) concentration. Determination of CO2 concentration is usually conducted by infrared gas analysis or chemical analysis (an alkali trap and subsequent titration, or soda lime absorption), and these methods were either unavailable or too time consuming for the number of samples analyzed. As a result, we have developed an analytical method for the determination of CO2 in gases using the gas chromatograph/mass spectrometer (GC-MS). This method requires a 5 μL injection, 5 minute analysis time, and the resulting figures of merit are comparable to most procedures present in the literature. Additionally, the microbial biomass carbon values obtained were consistent with previously published values in similar ecosystems.