Distinct roles for jasmonate synthesis and action in the systemic wound response of tomato
Distinct roles for jasmonate synthesis and action in the systemic wound response of tomato
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DOI：
10.1073/pnas.072072599
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发表时间：
2002-04-30
影响因子：
11.1
通讯作者：
Howe, GA
中科院分区：
文献类型：
--
作者：
Li, L;Li, CY;Howe, GA
植物对伤害和植食动物攻击的防御反应是由在受伤部位和未受损的远端叶片中操作的信号转导途径调节的。番茄中的遗传分析表明，系统素及其前体蛋白，前系统素，是创伤诱导的肠细胞信号通路的上游组分，该通路涉及茉莉酸（JA）的生物合成和作用。为了研究JA在系统信号传导中的作用，使用相互移植实验来分析JA生物合成突变体（spr-2）和JA响应突变体（jai-1）中蛋白酶抑制剂II基因的伤口诱导表达。结果表明，spr-2植物在产生嫁接传递的伤口信号方面有缺陷，但不识别。相反，jai-1植物在识别这种信号方面受到损害，但不会产生这种信号。它也被确定，嫁接传递的信号产生在砧木中的异位表达的prosystemin被识别的SPR-2，但不是由JAI-1接穗。两者合计，结果表明，响应于创伤或（亲）系统素的茉莉酸生物合成途径的激活所需的长距离信号的产生，其识别在远端叶片依赖于茉莉酸信号。这些发现表明，JA，或来自十八烷途径的相关化合物，可能作为一个可传递的伤口信号。
Plant defense responses to wounding and herbivore attack are regulated by signal transduction pathways that operate both at the site of wounding and in undamaged distal leaves. Genetic analysis in tomato indicates that systemin and its precursor protein, prosystemin, are upstream components of a wound-induced, entercellular signaling pathway that involves both the biosynthesis and action of jasmonic acid (JA). To examine the role of JA in systemic signaling, reciprocal grafting experiments were used to analyze wound-induced expression of the proteinase inhibitor II gene in a JA biosynthetic mutant (spr-2) and a JA response mutant (jai-1). The results showed that spr-2 plants are defective in the production, but not recognition, of a graft-transmissible wound signal. Conversely, jai-1 plants are compromised in the recognition of this signal but not its production. It was also determined that a graft-transmissible signal produced in response to ectopic expression of prosystemin in rootstocks was recognized by spr-2 but not by jai-1 scions. Taken together, the results show that activation of the jasmonate biosynthetic pathway in response to wounding or (pro)systemin is required for the production of a long-distance signal whose recognition in distal leaves depends on jasmonate signaling. These findings suggest that JA, or a related compound derived from the octadecanoid pathway, may act as a transmissible wound signal.