A novel enzyme immunoassay based on potentiometric measurement of molecular adsorption events by an extended-gate field-effect transistor sensor
A novel enzyme immunoassay based on potentiometric measurement of molecular adsorption events by an extended-gate field-effect transistor sensor
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DOI：
10.1016/j.bios.2007.01.011
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发表时间：
2007-06-15
影响因子：
12.6
通讯作者：
Shimoda, Maki
中科院分区：
文献类型：
--
作者：
Kamahori, Masao;Ishige, Yu;Shimoda, Maki
我们开发了一种基于分子吸附事件电位测量的新型酶免疫测定方法，该方法采用扩展栅场效应晶体管（FET）传感器。硫醇类化合物在金表面的吸附速率与该化合物的浓度有关。利用乙酰胆碱酯酶（AChE）生成巯基化合物的酶化学与酶联免疫吸附法（ELISA）相结合，构建了电化学酶免疫分析系统。在ache催化反应后，利用FET传感器检测生成的硫醇化合物在金电极上的吸附速率，从而得到抗原的量。在参比电极上叠加10khz或更高的高频电压时，测量稳定性得到改善。在参比电极上加1 MHz电压时，FET传感器可获得1 ~ 250 pg/mL白介素1 β对应的信号。基于FET传感器的酶联免疫吸附试验可以成功地检测到低至1 pg/mL浓度的白细胞介素1 β。(c) 2007 Elsevier BX版权所有
We developed a novel enzyme immunoassay based on a potentiometric measurement of molecular adsorption events by using an extended-gate field-effect transistor (FET) sensor. The adsorbing rate of a thiol compound on a gold surface was found to depend on the concentration of the compound. To construct an electrochemical enzyme immunoassay system by using the sensor, the enzyme chemistry of acetylcholinesterase (AChE) to generate a thiol compound was used and combined with the enzyme-linked immunosorbent assays (ELISA). After the AChE-catalyzed reaction, the amount of the antigen was obtained by detecting the adsorbing rate of the generated thiol compound on the gold electrode using the FET sensor. The measurement stability was also found to improve when a high frequency voltage of 10 kHz or more was superimposed to the reference electrode. The signal corresponding to a range between 1 and 250 pg/mL of Interleukin 1 beta was obtained by the FET sensor when a voltage of 1 MHz was superimposed onto the reference electrode. The FET sensor based ELISA used in this measurement technique can successfully detect Interleukin 1 beta at concentrations as low as 1 pg/mL. (c) 2007 Elsevier BX All rights reserved.