JUN promotes chicken female differentiation by inhibiting Smad2
JUN promotes chicken female differentiation by inhibiting Smad2
复制标题
JUN通过抑制Smad2促进鸡雌性分化
DOI：
10.1007/s10616-020-00447-y
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发表时间：
2021-01-02
期刊：
影响因子：
2.2
通讯作者：
Zuo, Qisheng
中科院分区：
文献类型：
--
作者：
Zhang, Ming;Xu, Pei;Zuo, Qisheng
The sex determination and control of poultry is a key problem in production and scientific research despite few studies on regulatory factors, especially transcription factors in sex determination. In the early stage of this study, high-throughput sequencing was used to screen the differentially expressed geneJUNin male and female embryonic stem cells (ESCs) and primordial germ cells (PGCs). The qRT-PCR discovered that the JUN gene significantly increased from embryonic days (E) 2.5 later in chicken embryo development, and the female gonad expression was much higher than that of the male after E14.5. Lentivirus shRNA-JUN, shRNA-Smad2interference, and OE-JUNoverexpression vectors were successfully constructed. After interfering withJUNin vivo, male characteristics appeared in ZW embryonic gonads at E18.5. Meanwhile, the male-specific genesDMRT1andSox9were upregulated, the female-specific genesFOXL2, ESR1,andCYP19A1were downregulated, and the estradiol in the gonads was significantly decreased. The situation was reversed after the overexpression ofJUN, ZZ chicken embryo developed into female sexual characteristics. The double luciferase report has found that theSmad2promoter activity was significantly upregulated after interference withJUN, and significantly increased after the deletion of theJUNbinding site. After the injection of theSmad2-shRNA vector into the blood vessel in vivo, it was discovered thatDMRT1andSox9of ZW embryos at E18.5 were downregulated,FOXL2andCYP19A1were significantly upregulated, and the gonads show femininity. In conclusion, this study proves thatJUNis a key regulator in the process of chicken female sex differentiation, which can inhibit the transcription ofSmad2and promote the synthesis of estradiol, and participate in the process of chicken sex differentiation. This study lays a foundation for the analysis of the molecular mechanism of chicken sex determination and the development of poultry sex control technology.
