DISTRIBUTION OF PROTEIN PHOSPHATASE INHIBITOR-1 IN BRAIN AND PERIPHERAL-TISSUES OF VARIOUS SPECIES - COMPARISON WITH DARPP-32
DISTRIBUTION OF PROTEIN PHOSPHATASE INHIBITOR-1 IN BRAIN AND PERIPHERAL-TISSUES OF VARIOUS SPECIES - COMPARISON WITH DARPP-32
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DOI：
10.1111/j.1471-4159.1992.tb08347.x
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发表时间：
1992-09-01
影响因子：
4.7
通讯作者：
GREENGARD, P
中科院分区：
文献类型：
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作者：
HEMMINGS, HC;GIRAULT, JA;GREENGARD, P
通过十二烷基硫酸钠-聚丙烯酰胺凝胶转移的免疫标记，使用特异性抗体，分析了抑制剂-1（一种环AMP调节的蛋白磷酸酶-1抑制剂）在不同物种的不同脑区和外周组织中的分布。将抑制剂-1的分布直接与DARPP-32的分布进行比较，DARPP-32是一种结构相关的蛋白磷酸酶-1的环状AMP调节抑制剂。在大鼠中枢神经系统中，M（r）30，000的单一免疫反应性蛋白（被鉴定为抑制剂-1）广泛分布。相反，DARPP-32高度集中在基底神经节。在来自青蛙（M（r）27，000）、海龟（M（r）29，000/33，000）、金丝雀（M（r）26，000）、鸽子（M（r）28，000）、小鼠（M（r）30，500）、兔（M（r）26，500）、牛（M（r）27，000）和猴（M（r）27，500）的脑组织中检测到抑制剂-1。但不是金鱼的在所研究物种的大多数外周组织中检测到不同水平的抑制剂-1;然而，在特定物种的某些组织中检测不到抑制剂-1（例如，大鼠和牛肝）。DARPP-32在除青蛙和金鱼外的所有测试物种的脑组织中检测到，但在大多数外周组织中未检测到。抑制剂-1和DARPP-32都集中在大鼠纹状体的胞浆和突触体胞浆中。抑制剂-1和DARPP-32在大鼠纹状体中的发育表达不同：抑制剂-1的水平在出生后第一周达到峰值，然后在出生后第三周下降，而DARPP-32的水平在出生后第三周增加到峰值水平，此后保持升高。由于抑制剂-1和DARPP-32在大鼠CNS中具有不同但部分重叠的区域分布和发育表达，并且在许多物种中具有不同的组织分布，因此它们的功能似乎不能完全互换。
The distribution of inhibitor-1, a cyclic AMP-regulated inhibitor of protein phosphatase-1, was analyzed in various brain regions and peripheral tissues of various species by immunolabeling of sodium dodecyl sulfate-polyacrylamide gel transfers using specific antibodies. The distribution of inhibitor-1 was directly compared to that of DARPP-32, a structurally related cyclic AMP-regulated inhibitor of protein phosphatase-1. In rat CNS, a single immunoreactive protein of M(r) 30,000, identified as inhibitor-1, was widely distributed. In contrast, DARPP-32 was highly concentrated in the basal ganglia. Inhibitor-1 was detected in brain tissue from frog (M(r) 27,000), turtle (M(r) 29,000/33,000), canary (M(r) 26,000), pigeon (M(r) 28,000), mouse (M(r) 30,500), rabbit (M(r) 26,500), cow (M(r) 27,000), and monkey (M(r) 27,500), but not from goldfish. Inhibitor-1 was detected at various levels in most peripheral tissues of the species studied; however, it was not detectable in certain tissues of particular species (e.g., rat and cow liver). DARPP-32 was detected in brain tissue of all the species tested except frog and goldfish, but was not detectable in most peripheral tissues. Both inhibitor-1 and DARPP-32 were concentrated in the cytosol and synaptosomal cytosol of rat striatum. The developmental expressions of inhibitor-1 and DARPP-32 in rat striatum differed: the level of inhibitor-1 peaked in the first postnatal week and then declined by the third postnatal week, whereas the level of DARPP-32 increased to a peak level by the third postnatal week and remained elevated thereafter. Because inhibitor-1 and DARPP-32 have distinct but partially overlapping regional distributions and developmental expression in rat CNS and have distinct tissue distributions in a number of species, it appears that their functions are not fully interchangeable.