Fluidic resistance control enables high-throughput establishment of mixed-species biofilms
Fluidic resistance control enables high-throughput establishment of mixed-species biofilms
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DOI：
10.2144/btn-2018-0150
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发表时间：
2019-05-01
期刊：
影响因子：
2.7
通讯作者：
Burmolle, Mette
中科院分区：
文献类型：
--
作者：
Hansen, Mads Frederik;Torp, Anders Meyer;Burmolle, Mette
细菌通常生活在混合物种的群落中，这些混合物种被嵌入由多糖、蛋白质和DNA组成的细胞外基质中，这种基质被称为生物膜。BioFlux微流控系统可用于研究不同介质中生物膜的形成。然而，分析流动中生物膜的结构和成熟需要适当的播种，这在处理不同大小的细菌、活动细菌或目标是大量复制时可能会证明是困难的。在这里，我们开发了一种有效的方案，利用粘度调节和播种指示染料来改善播种，并允许高通量检查和可视化流动中一致的单一和混合物种生物膜的发展。方法总结：在BioFlux微流控系统中，采用甘油和黑素分别增加培养基粘度和染色，建立了一种改进的细菌播种方案。这种新方案不会改变生物膜的发育，并允许在大量复制中形成一致的混合物种生物膜。
Bacteria often live in communities of mixed species embedded in a self-produced extracellular matrix of polysaccharides, proteins and DNA, termed biofilms. The BioFlux microfluidic flow system is useful for studying biofilm formation in different media under flow. However, analyzing the architecture and maturation of biofilms under flow requires a proper seeding, which can prove difficult when working with bacteria of different sizes, motile bacteria or aiming for a high number of replicates. Here we developed an efficient protocol that exploits viscosity tuning and seeding indicator dyes to improve seeding and allow for high-throughput examination and visualization of consistent mono- and mixed-species biofilm developments under flow.METHOD SUMMARY An improved protocol for seeding bacteria in the BioFlux microfluidic flow system was developed using glycerol and Nigrosin to increase the viscosity and stain the media, respectively. This new protocol does not alter biofilm development and allows consistent mixed-species biofilm formation in a copious numbers of replicates.