Isotopic comparison of gelatin extracted from bone powder with that from bone chunk and development of a framework for comparison of different extraction methods
Isotopic comparison of gelatin extracted from bone powder with that from bone chunk and development of a framework for comparison of different extraction methods
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DOI：
10.1016/j.jasrep.2016.11.044
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发表时间：
2017-02-01
影响因子：
1.6
通讯作者：
Yoneda, Minoru
中科院分区：
文献类型：
--
作者：
Tsutaya, Takumi;Gakuhari, Takashi;Yoneda, Minoru
从古代骨骼中提取胶原蛋白的稳定同位素分析已经成为一项常规工作。胶原蛋白可以从骨粉或骨块中提取，但很少有研究对这两种方法进行详细比较。本研究比较了从骨粉或骨块中提取的明胶的产率、元素浓度和碳氮稳定同位素比值，并提出了一种新的框架来比较不同提取方法从相同骨样品中提取的明胶样品的质量。研究人员使用了日本两处江户时代（公元1603-1867年）出土的40具成人骨骼。在“块状法”中，骨块经过脱矿、糊化和过滤得到明胶，而骨粉在“粉状法”中使用。块体法可显著提高20块保存完好的骨的明胶产率（+ 5.29 +/- 1.58%）、% C（+ 0.62 +/- 0.66%）和% N(+ 0.22 +/- 0.22%)，但C/N比（-0.01 +/- 0.03）、δ C-13（0.00 +/- 0.09）和δ N-15（-0.02 +/- 0.21）差异不显著。在20块保存较差的骨中，明胶产率（垂直为+ 1.27 ~ 1.33%）、% N（垂直为+ 0.41 ~ 0.49%）、C/N比（垂直为-0.07 ~ 0.07）和δ N-15（-0.12 +/- 0.37）变化显著，而% C（+ 0.37 +/- 1.11%）和δ C-13（-0.01 +/- 0.09）差异不显著。所观察到的显著性差异具有足够的统计能力（> 0.8），显著性水平为0.01（保存较差的骨骼d15N差异显著）。傅里叶变换红外光谱表明，提取的明胶主要由胶原蛋白组成，没有明显的污染物。一般来说，在古饮食研究的背景下，从保存良好和保存不良的骨骼中提取明胶的粉末和块状方法获得的d13C和d15N值没有实际差异。(C) 2016 Elsevier Ltd.版权所有。
Stable isotope analysis of collagen extracted from ancient bones has become a routine exercise. Collagen can be extracted from either bone powder or chunks, but little research has compared these two procedures in detail. In this study, yield, elemental concentration, and carbon and nitrogen stable isotope ratios of gelatin extracted from bone powder or chunks were compared, and a new framework to compare the quality of gelatin samples extracted from identical bone samples using different extraction methods was presented. Forty adult human skeletons from two sites in Japan dating from the Edo period (1603-1867 CE) were used. In the "chunk method," bone chunks are demineralized, gelatinized, and filtered to obtain gelatin, while bone powder is used in the " powder method." Using the chunk method, the gelatin yield (+ 5.29 +/- 1.58%), % C (+ 0.62 +/- 0.66%), and % N (+ 0.22 +/- 0.22%) significantly increased, but the C/N ratio (-0.01 +/- 0.03), delta C-13 (0.00 +/- 0.09), and delta N-15 (-0.02 +/- 0.21) did not significantly differ for 20 well-preserved bones. In 20 poorly preserved bones, the gelatin yield (+ 1.27 perpendicular to 1.33%), % N (+ 0.41 perpendicular to 0.49%), C/N ratio (-0.07 perpendicular to 0.07), and delta N-15 (-0.12 +/- 0.37) significantly changed, but the % C (+ 0.37 +/- 1.11%) and delta C-13 (-0.01 +/- 0.09) were not significantly different using the chunk method. The observed significant differences have a sufficient statistical power (> 0.8) with a significance level of 0.01 (except for the significant difference in d15N of poorly preserved bones). The Fourier transform infrared spectra indicate that the extracted gelatin mostly consists of collagen without apparent contaminants. In general, in the context of palaeodietary studies, there are no actual differences in the d13C and d15N values obtained from the powder and chunk methods for gelatin from well preserved as well as poorly preserved bones. (C) 2016 Elsevier Ltd. All rights reserved.