Lipid Dynamics due to Muscle Atrophy Induced by Immobilization
Lipid Dynamics due to Muscle Atrophy Induced by Immobilization
复制标题
DOI：
10.5650/jos.ess21045
复制
发表时间：
2021-01-01
影响因子：
1.5
通讯作者：
Goto-Inoue, Naoko
中科院分区：
文献类型：
--
作者：
Kimura, Keisuke;Morisasa, Mizuki;Goto-Inoue, Naoko
肌肉萎缩是指影响葡萄糖和脂质代谢的骨骼肌损失和功能障碍。此外，肌肉萎缩表现为癌症、糖尿病和肥胖症。在这项研究中，我们专注于肌肉萎缩过程中的脂质代谢。我们观察到腓肠肌与后肢关节制动8天的显著萎缩相关，并且无论肌纤维类型如何，都会发生肌肉萎缩。此外，我们使用薄层色谱法、液相色谱-质谱法和质谱成像法进行脂质分析。三酰甘油，磷脂酰丝氨酸和鞘磷脂的总量被发现在固定的肌肉中增加。此外，我们发现，磷脂酰丝氨酸，磷脂酰胆碱和鞘磷脂的特定分子种类增加固定。此外，脂肪甘油三酯脂肪酶的表达和环氧合酶-2的活性显着减少萎缩。从这些结果可以看出，在废用性肌肉萎缩期间发生脂质蓄积和特定脂肪酸的代谢变化。本研究具有验证肌肉萎缩的预防性治疗策略的意义。
Muscle atrophy refers to skeletal muscle loss and dysfunction that affects glucose and lipid metabolism. Moreover, muscle atrophy is manifested in cancer, diabetes, and obesity. In this study, we focused on lipid metabolism during muscle atrophy. We observed that the gastrocnemius muscle was associated with significant atrophy with 8 days of immobilization of hind limb joints and that muscle atrophy occurred regardless of the muscle fiber type. Further, we performed lipid analyses using thin layer chromatography, liquid chromatography-mass spectrometry, and mass spectrometry imaging. Total amounts of triacylglycerol, phosphatidylserine, and sphingomyelin were found to be increased in the immobilized muscle. Additionally, we found that specific molecular species of phosphatidylserine, phosphatidylcholine, and sphingomyelin were increased by immobilization. Furthermore, the expression of adipose triglyceride lipase and the activity of cyclooxygenase-2 were significantly reduced by atrophy. From these results, it was revealed that lipid accumulation and metabolic changes in specific fatty acids occur during disuse muscle atrophy. The present study holds implications in validating preventive treatment strategies for muscle atrophy.