Comparison of histological delineations of medial temporal lobe cortices by four independent neuroanatomy laboratories
Comparison of histological delineations of medial temporal lobe cortices by four independent neuroanatomy laboratories
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DOI：
10.1002/hipo.23602
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发表时间：
2024-02-28
期刊：
影响因子：
3.5
通讯作者：
Wisse，Laura E. M.
中科院分区：
文献类型：
--
作者：
Wuestefeld，Anika;Baumeister，Hannah;Wisse，Laura E. M.
内侧颞叶（MTL）皮层位于海马体附近，对记忆至关重要，并且易于积累某些神经病理学，如阿尔茨海默病神经元tau蛋白缠结。MTL皮层由几个功能和细胞结构特征不同的亚区组成。由于神经解剖学学派依赖于这些亚区的不同细胞结构定义，目前还不清楚他们对MTL皮质亚区的描述在多大程度上重叠。在这里，我们提供了一个概述的cytoarchitectonic定义的内嗅和海马旁皮质以及布罗德曼区（BA）35和36，提供了四个神经解剖学家从不同的实验室，旨在确定重叠和分歧划定的理由。尼氏染色系列是从三个人类标本（两个右半球和一个左半球）的颞叶中获得的。垂直于海马长轴制备切片（50 μm厚），横跨MTL皮质的整个纵向范围。四名神经解剖学家在间隔5 mm的数字化切片上注释了MTL皮质亚区（20倍放大率下像素大小为0.4 μm）。在神经解剖学家之间比较了包裹，术语和边界放置。每个亚区的Cytoarchitectonic功能进行了详细描述。注释的定性分析显示，内嗅皮层和BA35的定义具有较高的一致性，而BA36和海马旁皮层的定义在神经解剖学家中重叠较少。细胞结构定义的重叠程度部分反映在神经解剖学家对各自描述的一致性中。在结构之间的过渡区观察到较低的注释一致性，其中种子细胞结构特征表达不太显著。结果强调，MTL皮层的定义和包裹不同的神经解剖学学校，从而增加理解为什么这些差异可能会出现。这项工作为进一步推进人类MTL皮层的解剖学神经影像学研究奠定了重要基础。
The medial temporal lobe (MTL) cortex, located adjacent to the hippocampus, is crucial for memory and prone to the accumulation of certain neuropathologies such as Alzheimer's disease neurofibrillary tau tangles. The MTL cortex is composed of several subregions which differ in their functional and cytoarchitectonic features. As neuroanatomical schools rely on different cytoarchitectonic definitions of these subregions, it is unclear to what extent their delineations of MTL cortex subregions overlap. Here, we provide an overview of cytoarchitectonic definitions of the entorhinal and parahippocampal cortices as well as Brodmann areas (BA) 35 and 36, as provided by four neuroanatomists from different laboratories, aiming to identify the rationale for overlapping and diverging delineations. Nissl‐stained series were acquired from the temporal lobes of three human specimens (two right and one left hemisphere). Slices (50 μm thick) were prepared perpendicular to the long axis of the hippocampus spanning the entire longitudinal extent of the MTL cortex. Four neuroanatomists annotated MTL cortex subregions on digitized slices spaced 5 mm apart (pixel size 0.4 μm at 20× magnification). Parcellations, terminology, and border placement were compared among neuroanatomists. Cytoarchitectonic features of each subregion are described in detail. Qualitative analysis of the annotations showed higher agreement in the definitions of the entorhinal cortex and BA35, while the definitions of BA36 and the parahippocampal cortex exhibited less overlap among neuroanatomists. The degree of overlap of cytoarchitectonic definitions was partially reflected in the neuroanatomists' agreement on the respective delineations. Lower agreement in annotations was observed in transitional zones between structures where seminal cytoarchitectonic features are expressed less saliently. The results highlight that definitions and parcellations of the MTL cortex differ among neuroanatomical schools and thereby increase understanding of why these differences may arise. This work sets a crucial foundation to further advance anatomically‐informed neuroimaging research on the human MTL cortex.