Muscle contraction accelerates IL-6 mRNA expression in the rat masseter muscle
Muscle contraction accelerates IL-6 mRNA expression in the rat masseter muscle
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DOI：
10.1016/j.archoralbio.2006.10.025
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发表时间：
2007-05-01
影响因子：
3
通讯作者：
Kuboki, Takuo
中科院分区：
文献类型：
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作者：
Ono, Tsuyoshi;Maekawa, Kenji;Kuboki, Takuo
目的：:本研究旨在确定白细胞介素-6（IL-6）和白细胞介素-1 β（IL-1 β）（IL-1 β）mRNA的表达在对重复电刺激大鼠咬肌引起的肌肉收缩的反应中增加。方法：将体重140-160 g的雄性Wistar大鼠随机分为以下三组：电刺激（ES）组（n = 21）、角叉菜胶注射（CI）组（n = 24）、丹曲林钠（肌松药）注射（ESDI）+ES组（n = 7）。左侧咬肌进行ES或CI;右侧进行模拟ES或模拟CI。对两侧的肌肉组织取样用于总RNA分离。进行实时RT-PCR，以每个样品中的亲环素A（CypA）mRNA水平作为内部对照。平均相对IL-6（IL-6/cypA）和IL-1 β（IL-1 β/cypA）的mRNA水平进行了比较实验和模拟治疗的两侧在each group.Results：平均IL-6/CypA水平在ES-或Cl-治疗的肌肉显着增加，没有任何显着的增量变化，观察到在任何模拟治疗的肌肉。有趣的是，在ESDI组中，由ES引起的IL-6/cypA水平的增加被注射丹曲林钠抑制。此外，Cl处理的咬肌中的平均IL-1 β/cypA水平也显著增加，而在模拟处理的肌肉中没有观察到任何显著的增量变化。然而，有没有显着差异的ES和模拟治疗sides.Conclusions之间的咬肌的平均IL-1 β/cypA水平：：这些结果表明，IL-6 mRNA在大鼠咬肌的表达加速CI或ES诱导的重复肌肉收缩。由于IL-1 β（一种众所周知的促炎细胞因子）的mRNA水平没有被收缩改变，因此由肌肉收缩引起的IL-6 mRNA表达的加速似乎与局部炎症无关。(c)2006爱思唯尔有限公司版权所有。
Objective: : This study was conducted to determine if interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) mRNA expression increase in response to muscle contraction caused by repetitive electrical stimulation of the rat masseter muscle.Methods: : Male Wistar rats weighing 140-160 g were divided randomly into the following three groups: electrical stimulation (ES) group (n = 21), carrageenan injection (CI) group (n = 24), and ES under dantrolene sodium (muscle relaxant) injection (ESDI) group (n = 7). ES or CI was done to the left masseter; and mock ES or mock Cl to the right. Muscle tissues on both sides were sampled for total RNA isolation. Real-time RT-PCR was performed, with the cyclophilin A (CypA) mRNA level in each sample as an internal control. Mean relative IL-6 (il-6/cypA) and IL-1 beta (il-1 beta/cypA) mRNA levels were compared between the experimental and mock-treated sides within each group.Results: Mean IL-6/CypA levels in the ES- or Cl-treated muscle significantly increased, without any significant incremental change observed in either mock-treated muscle. Interestingly, the increase in the il-6/cypA level caused by the ES was suppressed by the injection of dantrolene sodium in the ESDI group. Furthermore, the mean il-1 beta/cypA level in the Cl-treated masseter also significantly increased without any significant incremental change observed in the mock-treated muscle. However, there was no significant difference in the mean il-1 beta/cypA levels in the masseter between the ES- and the mock-treated sides.Conclusions: : These results show that IL-6 mRNA expression in the rat masseter muscle was accelerated by the CI or by repetitive muscle contraction induced by ES. Since the mRNA level of IL-1 beta, a well-known proinflammatory cytokine, was not altered by the contraction, the accelerated IL-6 mRNA expression elicited by the muscle contraction does not seem to be related to local inflammation. (c) 2006 Elsevier Ltd. All rights reserved.