SURFACE-ANTIGEN IN EARLY DIFFERENTIATION
SURFACE-ANTIGEN IN EARLY DIFFERENTIATION
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DOI：
10.1073/pnas.74.10.4449
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
JACOB, F
中科院分区：
文献类型：
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作者：
KEMLER, R;BABINET, C;JACOB, F
将兔抗血清F9的Fab片段添加到2-细胞期小鼠胚胎的培养中，不会改变卵裂，但可以阻止致密桑椹胚和囊胚的形成。当将Fab片段添加到已经紧密的8细胞期甚至更老的桑椹胚中时，也观察到了类似的效果，但在胚腔形成开始时就消失了。这种效应是可逆的：不含Fab的松散的30-细胞胚胎在几个小时内变得致密，产生囊胚，重新植入假孕母亲体内就可以生出小鼠。二价抗F9抗体、针对其他胚胎表面抗原的血清Fab片段或琥珀酰刀豆球蛋白A不影响胚胎发育。
Addition of Fab fragments from rabbit antiserum to surface antigen F9 to 2-cell stage mouse embryos in culture did not alter cleavage; however, the addition prevented the formation of compact morulae and blastocysts. A similar effect was observed when Fab fragments were added to already compact 8-cell stage or even older morulae, but disappeared at the beginning of blastocoel formation. This effect was reversible: uncompact 30-cell embryos washed free of Fab became compact in a few hours, produced blastocysts and upon reimplantation into pseudopregnant mothers can produce mice. Development was not altered by divalent anti-F9 antibodies, by Fab fragments from sera directed against other embryo surface antigens or by succinyl concanavalin A.