Virus detection protocols for West Nile virus in vertebrate and mosquito specimens
Virus detection protocols for West Nile virus in vertebrate and mosquito specimens
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DOI：
10.1128/jcm.41.8.3661-3667.2003
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发表时间：
2003-08-01
影响因子：
9.4
通讯作者：
Kramer, LD
中科院分区：
文献类型：
--
作者：
Kauffman, EB;Jones, SA;Kramer, LD
最近在美国东北部和世界其他地区爆发的西尼罗河病毒（WNV）感染使得制定有效、敏感和快速的病毒监测方案变得至关重要。实验室检测是任何监测项目的支柱。自1999年以来，检测西尼罗河病毒存在的方案在灵敏度、速度、效率和特异性方面得到了改进。本文介绍了纽约州卫生部目前用于处理已提交给沃兹沃斯中心虫媒病毒实验室进行西尼罗河病毒检测的脊椎动物和蚊子标本的方案。
The recent outbreaks of West Nile virus (WNV) infection in the northeastern United States and other regions of the world have made it essential to develop efficient, sensitive, and rapid protocols for virus surveillance. Laboratory testing is the backbone of any surveillance program. Protocols to detect the presence of WNV have been refined since 1999 for sensitivity, speed, efficiency, and specificity. This paper presents the protocols currently used by the New York State Department of Health to handle vertebrate and mosquito specimens that have been submitted for WNV testing to the Arbovirus Laboratories of the Wadsworth Center.