Establishment of murine model of allergic photocontact dermatitis to ketoprofen and characterization of pathogenic T cells
Establishment of murine model of allergic photocontact dermatitis to ketoprofen and characterization of pathogenic T cells
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DOI：
10.1016/j.jdermsci.2005.08.006
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发表时间：
2006-02-01
影响因子：
4.6
通讯作者：
Tokura, Y
中科院分区：
文献类型：
--
作者：
Imai, S;Atarashi, K;Tokura, Y
背景：众所周知，当酮洛芬应用于皮肤并以紫外线A （UVA）照射时，会引起过敏性光接触性皮炎。目的：建立小鼠光敏性模型，并对与光敏性有关的致病性T细胞进行表征。方法：连续2天将酮洛芬涂于刮毛的腹部，并用UVA照射皮肤，致敏各品系小鼠。五天后，他们在耳垂上使用酮洛芬和UVA。采用RT-PCR方法对攻击部位的免疫淋巴结细胞和表皮细胞进行分析。结果：小鼠分别用4%和2%的酮洛芬加20 J/cm(2)的UVA致敏和激发成功。H-2(k)小鼠的反应高于其他菌株。将酮洛芬光敏H-2(k)小鼠的免疫淋巴结CD4（+）或CD8（+）细胞静脉转移给幼稚的同基因受体。接受CD4（+）而非CD8（+）细胞的小鼠表现出酮洛芬光敏性，但CD4（+）和CD8（+）细胞群的转移更有效。通过RT-PCR检测，光敏小鼠的淋巴结细胞表达高水平的Th2细胞因子（IL-4）和Th2趋化因子受体（CCR4）以及Th1细胞因子（ifn - γ）和Th1趋化因子受体（CXCR3） mRNA。此外，受损耳垂的表皮细胞表达Th1 （TARC）和Th2 （Mig）趋化因子水平升高。结论：认为Th1细胞和Th2细胞共同参与了酮洛芬致小鼠光接触性皮炎的发病机制。(c) 2005年日本皮肤病研究学会。爱思唯尔爱尔兰有限公司出版。版权所有。
Background: Ketoprofen is well known to evoke the allergic type of photocontact dermatitis when it is applied to the skin and irradiated with ultraviolet A (UVA) tight.Objective: We aimed to establish a murine model of this photosensitivity and to characterize pathogenic T cells concerned with the sensitivity.Methods: Various strains of mice were sensitized on two consecutive days by application of ketoprofen to the shaved abdomen and irradiation of the skin with UVA. Five days later, they were elicited with ketoprofen plus UVA on the earlobes. Immune Lymph node cells and epidermal cells from the challenged sites were analyzed by RT-PCR.Results: Mice were successfully sensitized and challenged with 4% and 2% ketoprofen, respective, plus UVA at20 J/cm(2). The responses in H-2(k) mice were higher than those in the other strains examined. Immune Lymph node CD4(+) or CD8(+) cells from ketoprofen-photosensitized H-2(k) mice were transferred i.v. to naive syngeneic recipients. Mice receiving CD4(+) but not CD8(+) cells exhibited ketoprofen photosensitivity, but transference of both CD4(+) and CD8(+) cell populations was more effective. Lymph node cells from photosensitized mice expressed high levels of mRNA for Th2 cytokine (IL-4) and Th2 chemokine receptor (CCR4) as well as Th1 cytokine (IFN-gamma) and Th1 chemokine receptor (CXCR3), as assessed by RT-PCR. In addition, epidermal cells from challenged earlobes expressed increased levels of both Th1 (TARC) and Th2 (Mig) chemokines.Conclusion: It is considered that not only Th1 but also Th2 cells participate in the pathogenesis of murine photocontact dermatitis to ketoprofen. (c) 2005 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. ALL rights reserved.