Exploring laccase genes from plant pathogen genomes: a bioinformatic approach
Exploring laccase genes from plant pathogen genomes: a bioinformatic approach
复制标题
DOI：
10.4238/2015.october.29.21
复制
发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Yu, X. M.
中科院分区：
文献类型：
--
作者：
Feng, B. Z.;Li, P. Q.;Yu, X. M.
迄今为止，漆酶的研究主要集中在植物和真菌漆酶及其目前在生物技术应用中的应用。相比之下，很少有人知道漆酶从植物病原体，虽然最近在全基因组测序越来越多的生物体的快速进展，促进了他们的识别和确定其起源。本研究通过对漆酶在细菌、真菌和卵菌中的分布进行比较分析，并通过比较它们的氨基酸组成，确定它们之间的关系。我们检索了20个公开的植物病原体基因组的漆酶基因。其中，125个漆酶基因被确定，包括7个在细菌基因组中，101个在真菌基因组中，17个在卵菌基因组中。这些基因的预测蛋白模型大多具有典型的真菌漆酶特征，具有4个保守结构域，其中1个半胱氨酸和10个组氨酸残基。系统发育分析表明，来自细菌和卵菌的漆酶可分为两个不同的分支，而真菌漆酶则可分为三个主要分支。这些结果为漆酶在植物病原菌中的作用提供了理论基础，并可用于指导未来对这些酶的研究。
To date, research on laccases has mostly been focused on plant and fungal laccases and their current use in biotechnological applications. In contrast, little is known about laccases from plant pathogens, although recent rapid progress in whole genome sequencing of an increasing number of organisms has facilitated their identification and ascertainment of their origins. In this study, a comparative analysis was performed to elucidate the distribution of laccases among bacteria, fungi, and oomycetes, and, through comparison of their amino acids, to determine the relationships between them. We retrieved the laccase genes for the 20 publicly available plant pathogen genomes. From these, 125 laccase genes were identified in total, including seven in bacterial genomes, 101 in fungal genomes, and 17 in oomycete genomes. Most of the predicted protein models of these genes shared typical fungal laccase characteristics, possessing four conserved domains with one cysteine and ten histidine residues at these domains. Phylogenetic analysis illustrated that laccases from bacteria and oomycetes were grouped into two distinct clades, whereas fungal laccases clustered in three main clades. These results provide the theoretical groundwork regarding the role of laccases in plant pathogens and might be used to guide future research into these enzymes.