Taurine and vitamin C modify monocyte and endothelial dysfunction in young smokers
Taurine and vitamin C modify monocyte and endothelial dysfunction in young smokers
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DOI：
10.1161/01.cir.0000046447.72402.47
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发表时间：
2003-01-28
期刊：
影响因子：
37.8
通讯作者：
Bouchier-Hayes, DJ
中科院分区：
文献类型：
--
作者：
Fennessy, FM;Moneley, DS;Bouchier-Hayes, DJ
背景-由单核细胞-内皮相互作用引发的内皮功能障碍先前已在许多血管病中观察到，包括慢性吸烟。牛磺酸（一种半必需氨基酸）和维生素 C（一种天然抗氧化剂）此前已被证明在受到促炎性损伤时具有内皮保护作用。因此，我们假设牛磺酸和维生素 C 可以通过改变单核细胞-内皮相互作用来恢复年轻吸烟者的内皮功能。方法和结果 - 使用双重超声检查在体内评估内皮依赖性血管舒张，并使用内皮细胞培养物（人脐静脉内皮细胞 [HUVEC]）和单核细胞条件培养基（MCM）在体外评估单核细胞-内皮相互作用。与不吸烟者相比，年轻吸烟者的内皮依赖性血管舒张功能显着受损。对年轻吸烟者使用 2 克/天的维生素 C 进行预处理 5 天，更显着的是，使用 1.5 克/天的牛磺酸减弱了这种反应。从吸烟者身上获取的 MCM 会损害一氧化氮的释放，并增加 HUVEC 中内皮素-1 的释放水平。当用牛磺酸治疗过的吸烟者的 MCM 培养 HUVEC 时，一氧化氮和内皮素-1 的水平恢复到对照水平。这是由于内皮一氧化氮合酶表达上调。结论-这些观察结果表明，牛磺酸补充对大血管内皮功能具有有益影响，并且有必要研究其对血脂异常状态下改变的内皮功能的影响。
Background-Endothelial dysfunction initiated by monocyte-endothelial interactions has previously been observed in many vasculopathies, including chronic cigarette smoking. Taurine, a semiessential amino acid, and vitamin C, a naturally occurring antioxidant, have previously been shown to have endothelial protective effects when exposed to proinflammatory insults. Therefore, we hypothesized that taurine and vitamin C would restore endothelial function in young smokers by modifying monocyte-endothelial interactions.Methods and Results-Endothelial-dependent vasodilatation was assessed in vivo using duplex ultrasonography, and monocyte-endothelial interactions were assessed in vitro using endothelial cell culture (human umbilical vein endothelial cells [HUVECs]) with monocyte-conditioned medium (MCM). Endothelial-dependent vasodilatation was significantly impaired in young smokers compared with nonsmokers. Pretreatment of young smokers for 5 days with 2 g/d vitamin C and, more significantly, with 1.5 g/d taurine attenuated this response. MCM taken from smokers impaired the release of nitric oxide and increased the levels of endothelin-1 release from HUVECs. When HUVECs were cultured with MCM from smokers who had been treated with taurine, the levels of nitric oxide and endothelin-1 returned toward control levels. This was attributed to an upregulation in endothelial nitric oxide synthase expression.Conclusions-These observations suggest that taurine supplementation has a beneficial impact on macrovascular endothelial function, and an investigation of its effect on altered endothelial function in dyslipidemic states is warranted.