Importance of the critical-size bone defect in testing bone-regenerating materials
Importance of the critical-size bone defect in testing bone-regenerating materials
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DOI：
10.1097/00001665-199807000-00004
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发表时间：
1998-07-01
影响因子：
0.9
通讯作者：
Vargervik, K
中科院分区：
文献类型：
--
作者：
Bosch, C;Melsen, B;Vargervik, K
人们对开发替代材料和人重组骨诱导因子以增强颅面复合体和骨骼其他部分的骨再生存在很大兴趣。一个持续的问题是找到一种动物模型，allo-tvs比较不同的骨促进材料。本研究的目的是：1）确定成年大鼠5 mm颅骨缺损是否符合临界尺寸骨缺损的要求; 2）讨论该动物模型在评估颅面区域骨修复中的用途。对56只56月龄Wistar大鼠的颅骨进行双侧全层颅骨缺损环钻术。除了少量的骨形成局限于缺损的边缘，没有一个缺损显示出任何迹象，自发性骨再生术后6个月和12个月。因此，全层5 mm颅骨缺损符合临界尺寸骨缺损的标准。该动物模型允许采用配对设计进行实验，它避免了在骨缺损中纳入矢状缝，从而通过降低损伤中矢状窦的风险将发病率降至最低。已建立了一个适当的实验模型来评价骨促进材料在颅颌面部骨缺损愈合中的效率。
A substantial interest exists in developing substitute materials and human recombinant bone-inducing factors to enhance bone regeneration both in the craniofacial complex and in other parts of the skeleton. A persistent problem has been to find an animal model that allo-tvs for the comparison of different osteopromotive materials. The purpose of this investigation was 1) to determine whether a 5-mm calvarial defect in adult rats fulfilled the requirements of a critical-size bone defect and 2) to discuss the use of this animal model in assessing bone repair in the craniofacial region. Bilateral full-thickness calvarial defects were trephined in the calvarial bone of 56 5- to 6-month-old Wistar rats. Except for minor amounts of bone formation limited to the margins of the defect, none of the defects revealed any signs of spontaneous bone regeneration 6 and 12 months after surgery. The full-thickness 5-mm calvarial defects thus fulfill the criteria for a critical-size bone defect. This animal model allows for an experiment with a paired design, it avoids inclusion of the sagittal suture in the osseous defect, and it thereby minimizes morbidity by reducing the risk of damaging the midsagittal sinus. An adequate experimental model has been developed to evaluate the efficiency of osteopromotive materials in the healing of bone defects in the craniomaxillofacial region.