Longitudinal Hemodynamic Changes Within the Optic Nerve Head in Experimental Glaucoma
Longitudinal Hemodynamic Changes Within the Optic Nerve Head in Experimental Glaucoma
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DOI：
10.1167/iovs.13-12013
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发表时间：
2013-06-01
影响因子：
4.4
通讯作者：
Wang, Lin
中科院分区：
文献类型：
--
作者：
Cull, Grant;Burgoyne, Claude F.;Wang, Lin
目的.目的观察实验性青光眼（EG）视神经乳头（ONH）基底血流量（BF）的纵向变化。用激光照射15只成年恒河猴的小梁网，造成单侧眼压升高。在激光治疗前后，每两周分别用光谱域光学相干断层扫描仪和激光散斑血流仪（LSFG）测量视网膜神经纤维层厚度（RNFLT）和ONH BF。EG眼的平均术后眼压为20.2 ± 5.9 mm Hg，对照眼为12.3 ± 2.6 mm Hg（P < 0.0001）。纵向变化基础ONH BF与RNFLT的变化密切相关，因为EG进展从早期到中晚期的损害，皮尔逊相关系数范围为0.64至0.97（平均= 0.81）和平均斜率为1.0。在早期阶段（RNFLT损失< 10%），基础ONH BF相对于基线和其他对照组轻度增加（9% +/-10%，P = 0.004）（P 0.02）。基础ONH BF在EG眼睛的后续阶段中持续下降，在研究的最后阶段达到基线以下25.0% +/-9.6%（P < 0.0001）（RNFLT损失> 40%）。在对侧对照眼中，基础ONH BF随时间推移无显着变化（P = 0.27）。结论。在基于慢性轻度至中度IOP升高的EG中，观察到ONH BF改变的两相模式。ONH BF在最早期增加（而RNFLT在基线的10%以内），随后线性下降，与RNFLT损失密切相关。
PURPOSE. To characterize longitudinal changes in basal blood flow (BF) of the optic nerve head (ONH) during progression of structural damage in experimental glaucoma (EG).METHODS. Unilateral elevation of IOP was induced in 15 adult rhesus macaques by laser treatment to the trabecular meshwork. Prior to and after laser, retinal nerve fiber layer thickness (RNFLT) and ONH BF were measured biweekly by spectral-domain optical coherence tomography and a laser speckle flowgraphy device (LSFG), respectively.RESULTS. Average postlaser IOP was 20.2 +/- 5.9 mm Hg in EG eyes and 12.3 +/- 2.6 mm Hg in control eyes (P < 0.0001). Longitudinal changes in basal ONH BF were strongly associated with changes in RNFLT as EG progressed from early through moderately advanced stages of damage, with Pearson correlation coefficients ranging from 0.64 to 0.97 (average = 0.81) and an average slope of 1.0. During early stage (RNFLT loss < 10%), basal ONH BF was mildly increased (9% +/- 10%, P = 0.004) relative to baseline and compared with fellow controls (P 0.02). Basal ONH BF declined continuously throughout subsequent stages in EG eyes reaching 25.0% +/- 9.6% (P < 0.0001) below baseline at the final stage studied (RNFLT loss > 40%). In fellow control eyes, there was no significant change in basal ONH BF over time (P = 0.27).CONCLUSIONS. In EG based on chronic mild-to-moderate IOP elevation, a two-phase pattern of ONH BF alteration was observed. ONH BF increased during the earliest stage (while RNFLT was within 10% of baseline) followed by a linear decline that was strongly correlated with loss of RNFLT.