Effects of two new siderophore-producing rhizobacteria on growth and iron content of maize and canola plants
Effects of two new siderophore-producing rhizobacteria on growth and iron content of maize and canola plants
复制标题
DOI：
10.1080/01904167.2016.1262409
复制
发表时间：
2017-01-01
影响因子：
2.1
通讯作者：
Besharati, Hossein
中科院分区：
文献类型：
--
作者：
Ghavami, Nazanin;Alikhani, Hossein Ali;Besharati, Hossein
产生铁载体的根际细菌通过向植物提供有效铁而有益地影响植物生长。本研究从油菜根际土壤中分离到细菌，并对其进行了初步研究。在伊朗中部地区种植的植物，因为存在产生铁载体的细菌。共45个不同的分离株被发现产生铁载体使用定性Chrome天青磺酸盐（CAS）琼脂测定。其中，10个分离物，根据最高的晕直径/菌落直径比，选择使用CAS-液体测定来量化铁载体生产率。使用多种生物化学测定来确定由十个分离物中的每一个产生的铁载体的类型。最好的分离株，根据生产率最高的异羟肟酸盐或羧酸盐，确定并用于进一步的研究。根据16 S rRNA基因序列分析和多种表型特征，将分离菌株鉴定为云南微球菌（Micrococcus yunnanensis YIM 65004（T））和螯噬寡养单胞菌（Stenotrophomonas chelatiphaga LPM-5（T））。我们还研究了最有前途的菌株（YIM 65004和LPM-5）在温室条件下对油菜和玉米植株的植物生长促进作用。本研究结果表明，M. yunnanensis和S.与对照相比，螯合噬菌体显著增加了根和地上部的增重和铁（Fe）含量，表明这些根际细菌对植物的生长和发育具有有益的影响。本研究报道了M. yunnanensis和S. chelatiphaga为伊朗新记录。后者是首次报道的植物（油菜）根际。此外，M. yunnanensis和S. chelatiphaga产生铁载体的研究首次被记录。
Siderophore-producing rhizobacteria beneficially affect plant growth by providing available iron to plants. In this study, bacteria were isolated from the rhizosphere of canola (Brassica napus L.) plants grown in the central fields in Iran, for the presence of siderophore-producing bacteria. A total of 45 distinct isolates were found to produce siderophore using qualitative chrome azurol sulfonate (CAS)-agar assay. Of them, ten isolates, based on the highest halo diameter/colony diameter ratios, were selected to quantify the rate of siderophore production using CAS-liquid assay. A variety of biochemical assays was used to determine the type(s) of siderophores produced by each of the ten isolates. The best isolates, based on production of the highest rates of either hydroxamates or carboxylates, were identified and used in further studies. Based on 16S ribosomal ribonucleic acid (rRNA) sequence analysis and a variety of phenotypic properties, the isolates were identified as Micrococcus yunnanensis YIM 65004 (T) and Stenotrophomonas chelatiphaga LPM-5 (T). We also studied the plant growth-promoting effect of the most promising isolates (YIM 65004 and LPM-5) on canola and maize plants under greenhouse conditions. The results of this study showed that M. yunnanensis and S. chelatiphaga increased gain weight and iron (Fe) content of roots and shoots significantly, in comparison with control, indicating beneficial effects of these rhizobacteria on plant growth and development. This study reports M. yunnanensis and S. chelatiphaga, as new records for Iran. The latter is reported for the first time from plant (canola) rhizosphere. Besides, the ability of both M. yunnanensis and S. chelatiphaga to produce siderophores is documented for the first time.