Ng-MIP, a surface-exposed lipoprotein of Neisseria gonorrhoeae, has a peptidyl-prolyl cis/trans isomerase (PPIase) activity and is involved in persistence in macrophages
Ng-MIP, a surface-exposed lipoprotein of Neisseria gonorrhoeae, has a peptidyl-prolyl cis/trans isomerase (PPIase) activity and is involved in persistence in macrophages
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DOI：
10.1111/j.1365-2958.2005.04859.x
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发表时间：
2005-11-01
影响因子：
3.6
通讯作者：
Pizza, M
中科院分区：
文献类型：
--
作者：
Leuzzi, R;Serino, L;Pizza, M
巨噬细胞感染性增强因子（MIP）是军团菌、衣原体和锥虫等细胞内微生物的表面暴露毒力因子家族。这些蛋白质显示肽基脯氨酰顺式/反式异构酶（PPI酶）活性，其被免疫抑制剂FK 506和雷帕霉素抑制。在这里，我们描述了鉴定和表征淋病奈瑟菌的Ng-MIP，一种表面暴露的脂蛋白与MIP具有高度同源性。该蛋白质是具有雷帕霉素抑制的PPI酶活性的同源二聚体，证实其是MIP家族的功能性成员。通过缺失N.淋病F62菌株，评价其在小鼠和人巨噬细胞感染中的作用。我们发现Ng-MIP促进了N.巨噬细胞中的淋病，突出了这种蛋白质在促进淋球菌感染的持续性中的可能作用。
Macrophage infectivity potentiators (MIPs) are a family of surface-exposed virulence factors of intracellular microorganisms such as Legionella, Chlamydia and Trypanosoma. These proteins display peptidyl-prolyl cis/trans isomerase (PPIase) activity that is inhibited by immunosuppressants FK506 and rapamycin. Here we describe the identification and characterization in Neisseria gonorrhoeae of Ng-MIP, a surface-exposed lipoprotein with high homology to MIPs. The protein is an homodimer with rapamycin-inhibited PPIase activity confirming that it is a functional member of the MIP family. A knock-out strain, generated by deletion of the mip gene in N. gonorrhoeae F62 strain, was evaluated for its role in infection of mouse and human macrophages. We show that Ng-MIP promotes the intracellular survival of N. gonorrhoeae in macrophages, highlighting a possible role of this protein in promoting the persistence of gonococcal infection.