A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep
A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep
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DOI：
10.1111/tbed.12623
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发表时间：
2017-06-01
影响因子：
4.3
通讯作者：
Beer, M.
中科院分区：
文献类型：
--
作者：
Pfaff, F.;Schlottau, K.;Beer, M.
2013年6月，一只4岁的威尔士山母羊和2014年3月同一品种和同一羊群的一只10天大的羔羊出现了进行性神经系统体征，包括感觉抑制、震颤和异常行为。脑和脊髓的神经病理学检查检测到非化脓性脊髓灰质炎脑脊髓炎和背根神经节炎，这是两只绵羊中嗜神经病毒剂的特征。对这两种动物不同组织样本的宏基因组分析鉴定了一种新型绵羊星状病毒（OvAstV）。通过RT-qPCR证实中枢神经系统中存在病毒基因组。虽然这些病例相隔9个月，但所鉴定的OvAstV具有几乎相同的序列，在整个基因组中仅在三个核苷酸位置上存在差异。系统发育分析表明OvAstV与嗜神经牛星状病毒和肠道OvAstV有密切关系。总之，这是首次报道的与脑炎和神经节炎相关的国内绵羊星状病毒感染病例。
In June 2013, a 4-year-old Welsh Mountain ewe and in March 2014 a 10-day-old lamb of the same breed and the same flock presented progressive neurological signs including depressed sensorium, tremor, and unusual behaviour. Neuropathological examination of the brain and spinal cord detected non-suppurative polioencephalomyelitis and dorsal root ganglionitis, characteristic of a neurotropic viral agent in both sheep. Metagenomic analysis of different tissue samples from both animals identified a novel Ovine Astrovirus (OvAstV). The presence of viral genome in the central nervous system was confirmed by RT-qPCR. Although the cases presented nine months apart, the identified OvAstV shared nearly identical sequences, differing in only three nucleotide positions across the complete genome. Phylogenetic analysis revealed a close relation of OvAstV to neurotropic bovine astroviruses and an enteric OvAstV. In conclusion, these are the first reported cases of astrovirus infection in domestic sheep that were associated with encephalitis and ganglionitis.