Selection of guide sequences that direct efficient cleavage of mRNA by human ribonuclease P.
Selection of guide sequences that direct efficient cleavage of mRNA by human ribonuclease P.
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DOI：
10.1126/science.8122108
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发表时间：
1994-03
期刊：
影响因子：
56.9
通讯作者：
Yan Yuan;S. Altman
中科院分区：
文献类型：
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作者：
Yan Yuan;S. Altman
任何RNA，当在与另一个寡核糖核苷酸称为外部指导序列（EGS）的复合物，可以成为核糖核酸酶P的底物。体外进化模拟被用来选择EGS，紧密结合到一个目标底物信使RNA，并增加切割效率的目标由人核糖核酸酶P的水平等于与天然底物实现。最有效的EGSs与靶RNA形成转移RNA转录因子样结构，其中反密码子茎的类似物已被破坏，这表明选择核糖核酸酶P的最佳底物产生不同于当今转移RNA前体的RNA结构。
Any RNA, when in a complex with another oligoribonucleotide known as an external guide sequence (EGS), can become a substrate for ribonuclease P. Simulation of evolution in vitro was used to select EGSs that bind tightly to a target substrate messenger RNA and that increase the efficiency of cleavage of the target by human ribonuclease P to a level equal to that achieved with natural substrates. The most efficient EGSs form transfer RNA precursor-like structures with the target RNA, in which the analog of the anticodon stem has been disrupted, an indication that selection for the optimal substrate for ribonuclease P yields an RNA structure different from that of present-day transfer RNA precursors.