Dexmedetomidine protects against lipid peroxidation and erythrocyte deformability alterations in experimental hepatic ischemia reperfusion injury
Dexmedetomidine protects against lipid peroxidation and erythrocyte deformability alterations in experimental hepatic ischemia reperfusion injury
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DOI：
10.3402/ljm.v7i0.18185
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发表时间：
2012-01-01
影响因子：
2.4
通讯作者：
Yaylak, Faik
中科院分区：
文献类型：
--
作者：
Arslan, Mustafa;Comu, Faruk Metin;Yaylak, Faik
背景：肝脏缺血再灌注损伤是肝脏外科手术和移植中常见的临床问题。在这种损伤中可以观察到几种细胞和组织结构和功能的变化。本研究旨在探讨右旋美托咪定对大鼠缺血再灌注损伤时脂质过氧化和红细胞变形能力的影响。方法：24只Wistar大鼠随机分为对照组(C)、缺血再灌注损伤组(I/R)和右美托咪胺组(I/R-D)。门静脉阻断45min诱导缺血，再灌注期为45min。I/R-D组：右美托咪定100 mg/kg，ip。门静脉阻断前30分钟。结果：肝缺血再灌注组血清丙二醛和丙二醛活性明显升高，红细胞变形性指数明显降低。然而，在门静脉阻断前给予右美托咪定可以预防这些改变。结论：这些发现清楚地表明红细胞变形性指数在肝脏缺血再灌注损伤中降低，并具有潜在的预防作用。右旋美托咪定对肝脏I/R损伤的保护作用也是通过减少脂质过氧化来实现的。进一步的实验和临床研究可能会阐明这些发现的分子机制和临床意义。
Background: Hepatic ischemia-reperfusion injury is a common clinical problem in hepatic surgery and transplantation. Several cellular and tissue structural and functional alterations are observed in such injury. The aim of this study was to evaluate the effect of dexmedetomidine on lipid peroxidation and erythrocyte deformability during ischemia-reperfusion injury in rats.Methods: Twenty-four Wistar Albino rats were randomly separated into three groups as control (C), ischemia-reperfusion injury (I/R) and dexmedetomidine group (I/R-D). Ischemia was induced with portal clampage for 45 min and reperfusion period was 45 min after declampage. Group I/R-D received dexmedetomidine 100 mu g/kg i.p. 30 min before portal clampage. Serum malondialdehyde and superoxide dismutase activities to document lipid peroxidation and erythrocyte deformability index were investigated.Results: Serum superoxide dismutase and malondialdehyde activity levels were significantly higher and erythrocyte deformability index was decreased in hepatic ischemia-reperfusion group. However, these changes were observed to be prevented with dexmedetomidine treatment when given before portal clampage.Conclusion: These findings clearly indicate that erythrocyte deformability index is decreased in hepatic ischemia reperfusion injury and has a potential role to prevent these alterations. The protective effect of dexmedetomidine on hepatic I/R injury is also decreased lipid peroxidation. Further experimental and clinical investigations may clarify the molecular mechanisms and clinical significance of these findings.